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Titolo:
Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle
Autore:
Yasui, A; Oka, K; Usunomiya, H; Kakudo, K; Suzuki, T; Yoshida, T; Park, HM; Fukazawa, K; Muramatsu, T;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Mol Biosci, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 osci, Nagoya, Aichi 4648601, Japan Chubu Natl Hosp, Dept Surg, Obu 4748511, Japan Chubu Natl Hosp Obu Japan4748511 tl Hosp, Dept Surg, Obu 4748511, Japan Nagoya Univ, Grad Sch Med, Dept Surg, Nagoya, Aichi 4668550, Japan Nagoya Univ Nagoya Aichi Japan 4668550 Surg, Nagoya, Aichi 4668550, Japan Wakayama Med Coll, Dept Pathol 2, Wakayama 6408155, Japan Wakayama Med Coll Wakayama Japan 6408155 thol 2, Wakayama 6408155, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 5, volume: 8, anno: 2001,
pagine: 489 - 494
SICI:
1107-3756(200111)8:5<489:EGSBIV>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VIVO; LAYING HENS; EXPRESSION; ERYTHROPOIETIN; TRANSFECTION; CELLS; INJECTION; EMBRYOS; OVIDUCT; MICE;
Keywords:
skeletal muscle; in vivo electroporation; gastrin secretion; GFP expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Muramatsu, T Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Mol Biosci, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 Aichi 4648601, Japan
Citazione:
A. Yasui et al., "Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle", INT J MOL M, 8(5), 2001, pp. 489-494

Abstract

Whether or not in vivo acne transfer of gastrin gene into skeletal muscle by electroporation could modify gastrin secretion was examined. The expression plasmid vector, either pMEPrGaspA encoding the rat gastrin acne or pEGFP-N1 encoding the GFP reporter gene was injected into M. rectus abdominis bf rats or M. biceps formis of mice. Subsequently, square electric pulses ofdirect current were applied six times at 25 V with a loading period of 100msec per pulse. Clear foreign gene expression in the skeletal muscle was demonstrated by. both GFP fluorescence and immunostaining of rat gastrin. Time course changes in plasma gastrin levels after transfection revealed thatin rats, gastrin gene transfer significantly increased the plasma gastrin level for 4 weeks post-transfection (P<0.05), but the difference diminishedat the end of the 10-week period. In mice, plasma gastrin level elevated similarly for 3 weeks, and pH of gastric contents decreased in the gastrin gene transfected group compared with the control counterpart (P<0.05). Thesefindings suggest that localized in vivo gene transfer by electroporation allows skeletal muscle to become an artificial endocrine tissue for hormonalmanipulation of animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 07:25:15