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Titolo:
Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase 2 in human peripheral blood leukocytes
Autore:
Franchina, M; Hooper, J; Kay, PH;
Indirizzi:
Univ Western Australia, Dept Pathol, Mol Pathol Lab, Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 nds, WA 6907, Australia
Titolo Testata:
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
fascicolo: 11, volume: 33, anno: 2001,
pagine: 1104 - 1115
SICI:
1357-2725(200111)33:11<1104:FNASTO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA SECONDARY STRUCTURE; SINGLE AMINO-ACID; MESSENGER-RNA; METHYLATION; GENE; BINDING; TISSUES; CELLS; YEAST;
Keywords:
DNA methylation; splice variant; consensus splicing signals; RNA secondary structure; methyltransferase motifs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Kay, PH Univ Western Australia, Dept Pathol, Mol Pathol Lab, Nedlands, WA 6907, Australia Univ Western Australia Nedlands WA Australia 6907 6907, Australia
Citazione:
M. Franchina et al., "Five novel alternatively spliced transcripts of DNA (cytosine-5) methyltransferase 2 in human peripheral blood leukocytes", INT J BIO C, 33(11), 2001, pp. 1104-1115

Abstract

Alternative splicing of RNA molecules transcribed from DNA (cytosine-5) methyltransferases has been proposed as a mechanism by which methylation is able to effect diverse biological processes in higher eukaryotes. This studyhas investigated transcriptional versatility of DNA (cytosine-5) methyltransferase 2, which may methylate cytosine residues within 5'-CCTGG-3' pentanucleotides in regions Of the human genome devoid of 5'-CG-3' methylation. Five novel splice variants of DNA (cytosine-5) methyltransferase 2 were identified in the peripheral blood leukocytes of healthy subjects following cloning and sequencing of RT-PCR products amplified using gene specific oligodcoxyribonucleotide primers. The generation of some of these splice variantsmay be influenced by the formation of secondary structures within pre-mRNAdue to the repetition of sequences flanking alternatively spliced exons ina reverse and complementary orientation on the same strand. These findingsenable novel approaches to investigate the role of RNA secondary structures in alternative splicing. The DNA (cytosine-5) methyltransferase 2 splice variants are generated in all the major cell types of peripheral blood, as well as in neoplastic lymphoid cells indicating that they are unlikely to generate proteins involved in control of the cell cycle or cellular differentiation. Interestingly, the gene products generated by some splice variantscompletely or partially lack highly conserved amino acid motifs shown to be important for the catalysis of cytosine methylation. The possibility cannot be excluded, therefore, that alternative splicing of DNA (cytosine-5) methyltransferase 2 pre-mRNA may generate protein isoforms which have different methylating capabilities or which are involved in biological processes other than the catalysis of cytosine methylation. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:03:59