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Titolo:
Molecular enhancement of porcine cardiac chronotropy
Autore:
Edelberg, JM; Huang, DT; Josephson, ME; Rosenberg, RD;
Indirizzi:
Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USA Beth Israel Deaconess Med Ctr Boston MA USA 02215 d, Boston, MA 02215 USA MIT, Dept Biol, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139MIT, Dept Biol, Cambridge, MA 02139 USA
Titolo Testata:
HEART
fascicolo: 5, volume: 86, anno: 2001,
pagine: 559 - 562
SICI:
1355-6037(200111)86:5<559:MEOPCC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SICK SINUS SYNDROME; IN-VITRO; CHAGAS-DISEASE; GENE-TRANSFER; EXPRESSION; HEART; MYOCARDIUM; MYOCYTES; THERAPY; INVIVO;
Keywords:
sinus node; adrenergic receptor; chronotropic agents; conduction system; gene therapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Edelberg, JM Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA 02215 USABeth Israel Deaconess Med Ctr Boston MA USA 02215 02215 USA
Citazione:
J.M. Edelberg et al., "Molecular enhancement of porcine cardiac chronotropy", HEART, 86(5), 2001, pp. 559-562

Abstract

Objective-To test the potential of gene transfer approaches to enhance cardiac chronotropy in a porcine system as a model of the human heart. Methods-Plasmids encoding either the human beta (2) adrenergic receptor orcontrol constructs were injected into the right atria of native Yorkshire pig hearts. Percutaneous electrophysiological recording catheters equipped with 33 gauge circular injection needles were positioned in the mid-lateralright atrium. At the site of the earliest atrial potential the circular injection needles were rotated into the myocardium and the beta (2) adrenergic receptor (n = 6) or control plasmid constructs (n = 5) were injected. Results-Injection of the beta (2) adrenergic receptor construct significantly enhanced chronotropy compared with control injections. The average (SD)heart rate of the pigs was 108 (16) beats/min before injection. Two days after injection with control plasmids the heart rate was 127 (25) beats/min (NS compared with preinjection rates). After injection with plasmid encoding the beta (2) adrenergic receptor the heart rate increased by 50% to 163 (33) beats/min (p < 0.05 compared with preinjection and postinjection control rates). Conclusions-The present studies showed in a large animal model that local targeting of gene expression may be a feasible modality to regulate cardiacpacemaking activity. In addition, these investigations provide an experimental basis for developing future clinical gene transfer approaches to upregulate heart rate and modulate cardiac conduction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/01/20 alle ore 12:56:31