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Titolo:
Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells
Autore:
Guo, S; Sato, T; Shirane, K; Furukawa, K;
Indirizzi:
Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, Itabashi Ku, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 okyo 1730015, Japan Beijing Med Univ, Dept Pathol, Beijing 100083, Peoples R China Beijing MedUniv Beijing Peoples R China 100083 100083, Peoples R China Nisshin Flour Milling Co Ltd, Saitama 3568511, Japan Nisshin Flour MillingCo Ltd Saitama Japan 3568511 aitama 3568511, Japan
Titolo Testata:
GLYCOBIOLOGY
fascicolo: 10, volume: 11, anno: 2001,
pagine: 813 - 820
SICI:
0959-6658(200110)11:10<813:GONOBH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-4-GALACTOSYLTRANSFERASE GENE FAMILY; BOVINE-MILK GALACTOSYLTRANSFERASE; UDP-GALACTOSE; INSECT CELLS; ACETYLLACTOSAMINE SYNTHESIS; MEMBRANE-GLYCOPROTEINS; POLYSIALIC ACID; CDNA CLONING; GLYCANS; PURIFICATION;
Keywords:
acceptor specificity; in vivo beta-1,4-galactosylation; K-m value; N-glycan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Tokyo Metropolitan Inst Gerontol, Dept Biosignal Res, ItabashiKu, Tokyo 1730015, Japan Tokyo Metropolitan Inst Gerontol Tokyo Japan 1730015 , Japan
Citazione:
S. Guo et al., "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells", GLYCOBIOLOG, 11(10), 2001, pp. 813-820

Abstract

Several studies showed that Sf-9 cells can synthesize the galactosylated N-linked oligosaccharides if beta -1,4-galactosyltransferase (beta -1,4-GalT) is supplied. The full-length human beta -1,4-GalT I, II, III, IV, V, and VI cDNAs were independently transfected into Sf-9 cells, and the galactosylation of endogenous membrane glycoproteins was examined by lectin blot analysis using Ricinus communis agglutinin-I (RCA-I), which preferentially interacts with oligosaccharides terminated with Gal beta1 --> 4GlcNAc group. Several RCA-I-reactive bands appeared in all of the gene-transfected cells, and disappeared on treatment of blots with beta -1,4-galactosidase or N-glycanase prior to incubation with lectin. Introduction of the antisense beta -1,4-GalT II and V cDNAs separately into human colorectal adenocarcinoma SW480 cells, in which beta -1,4-GalT I, II, and V genes were expressed, resulted in the reduction of RCA-I binding toward N-linked oligosaccharides of the membrane glycoproteins. Differences were found in their K-m. values toward UDP-Gal and GlcNAc beta -S-pNP and in their acceptor specificities towardoligosaccharides with the GlcNAc beta1 -->4(GlcNAc beta1 -->2)Man branch and with the GlcNAc beta1 -->6(GlcNAc beta1 -->2)Man branch. These results indicate that beta -1,4-GalTs II, III, IV, V, and VI are involved in the N-linked oligosaccharide biosynthesis cooperatively but not in a redundanat manner with beta -1,4-GalT I within cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 22:47:17