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Titolo:
Interaction of fission yeast ORC with essential adenine/thymine stretches in replication origins
Autore:
Takahashi, T; Masukata, H;
Indirizzi:
Osaka Univ, Grad Sch Sci, Dept Biol, Osaka 5600043, Japan Osaka Univ Osaka Japan 5600043 Sch Sci, Dept Biol, Osaka 5600043, Japan Japan Sci & Technol Corp, PRESTO, Kawaguchi, Japan Japan Sci & Technol Corp Kawaguchi Japan Corp, PRESTO, Kawaguchi, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 10, volume: 6, anno: 2001,
pagine: 837 - 849
SICI:
1356-9597(200110)6:10<837:IOFYOW>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHORION GENE AMPLIFICATION; BETA-GLOBIN LOCUS; DNA-REPLICATION; SCHIZOSACCHAROMYCES-POMBE; RECOGNITION COMPLEX; ARS ELEMENTS; S-PHASE; BUDDING YEAST; MCM PROTEINS; INITIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Masukata, H Osaka Univ, Grad Sch Sci, Dept Biol, 1-1 Machikaneyama Cho, Osaka 5600043,Japan Osaka Univ 1-1 Machikaneyama Cho Osaka Japan 5600043 43,Japan
Citazione:
T. Takahashi e H. Masukata, "Interaction of fission yeast ORC with essential adenine/thymine stretches in replication origins", GENES CELLS, 6(10), 2001, pp. 837-849

Abstract

Background: Eukaryotic DNA replication is initiated from distinct regions on the chromosome. However, the mechanism for recognition of replication origins is not known for most eukaryotes. In fission yeast, replication origins are isolated as autonomously replicating sequences (ARSs). Multiple adenine/thymine clusters are essential for replication, but no short consensus sequences are found. In this paper, we examined the interaction of adenine/thymine clusters with the replication initiation factor ORC. Results: The SpOrc1 or SpOrc2 immunoprecipitates (IPs) containing at leastfour subunits of SpORC, interacted with the ars2004 fragment, which is derived from a predominant replication origin on the chromosome. SpORC-IPs preferentially interacted with two regions of the ars2004, which consist of consecutive adenines and AAAAT repeats and are essential for ARS activity Thenucleotide sequences required for the interaction with SpORC-IPs correspond closely to those necessary for in vivo ARS activity. Conclusion: Our results suggest that the SpORC interacts with adenine/thymine stretches, which have been shown to be the most important component in the fission yeast replication origin. The presence of multiple SpORC-binding sites, with certain sequence variations, is characteristic for the fission yeast replication origins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:48:12