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Titolo:
Expression profile of active genes in human periodontal ligament and isolation of PLAP-1, a novel SLRP family gene
Autore:
Yamada, S; Murakami, S; Matoba, R; Ozawa, Y; Yokokoji, T; Nakahira, Y; Ikezawa, K; Takayama, S; Matsubara, K; Okada, H;
Indirizzi:
Osaka Univ, Grad Sch Dent, Dept Periodontol, Suita, Osaka 5650871, Japan Osaka Univ Suita Osaka Japan 5650871 odontol, Suita, Osaka 5650871, Japan Nara Inst Sci & Technol, Taisho Lab Funct Genom, Nara 6300101, Japan Nara Inst Sci & Technol Nara Japan 6300101 ct Genom, Nara 6300101, Japan
Titolo Testata:
GENE
fascicolo: 2, volume: 275, anno: 2001,
pagine: 279 - 286
SICI:
0378-1119(20010919)275:2<279:EPOAGI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RICH REPEAT PROTEINS; EXTRACELLULAR-MATRIX; COMPLEMENTARY-DNA; III COLLAGEN; GROWTH; CELLS;
Keywords:
3 '-directed cDNA library; gene signature; biglycan; decorin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Murakami, S Osaka Univ, Grad Sch Dent, Dept Periodontol, 1-8 Yamadaoka, Suita, Osaka 5650871, Japan Osaka Univ 1-8 Yamadaoka Suita Osaka Japan 5650871 0871, Japan
Citazione:
S. Yamada et al., "Expression profile of active genes in human periodontal ligament and isolation of PLAP-1, a novel SLRP family gene", GENE, 275(2), 2001, pp. 279-286

Abstract

Periodontal ligament (PDL) is one of the most important tissues in maintaining the homeostasis of tooth and tooth-supporting tissue, periodontium. Inthis study, we investigated the expression profile of active genes in the human PDL obtained by collecting sequences with a 3'-directed cDNA library,which faithfully represents the composition of the mRNA population. We succeeded in obtaining a total of 1752 cDNA sequences by sequencing randomly selected clones and identified a total of 1318 different species as gene signatures (GS) by their sequence identity, 344 of which were known genes in the GenBank, and 974 of which were new genes. The resulting expression profile showed that collagen type land type III were the most abundant genes andthat osteogenesis-related proteins, such as SPARC/osteonectin and osteoblast specific factor 2, were highly expressed. By comparing the expression profile of PDL with 44 profiles similarly obtained with unrelated human cell/tissue, nine novel genes, which are probably expressed specifically in PDL,were discovered. Among them, we cloned a full-length cDNA of GS5096, whichis frequently expressed in freshly-isolated periodontal tissue. We found that it encodes a novel protein, which is a new member of the class I small leucine-rich repeat proteoglycan family, and designated it PLAP-1 (periodontal ligament associated protein-1). PLAP-1 mRNA expression was confirmed inin vitro-maintained PDL cells and was enhanced during the course of the cytodifferentiation of the PDL cells into mineralized tissue-forming cells such as osteoblasts and cementoblasts. These findings suggest the involvementof PLAP-1 in the mineralized matrix formation in PDL tissues. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 19:25:33