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Titolo:
Effect of iron and manganese on hydroxyl radical production by 6-hydroxydopamine: Mediation of antioxidants
Autore:
Mendez-Alvarez, E; Soto-Otero, R; Hermida-Ameijeiras, A; Lopez-Martin, ME; Labandeira-Garcia, JL;
Indirizzi:
Univ Santiago de Compostela, Fac Med, Dept Bioquim & Biol Mol, Grp Neuroquim, E-15782 Santiago De Compostela, Spain Univ Santiago de Compostela Santiago De Compostela Spain E-15782 , Spain Univ Santiago de Compostela, Fac Med, Dept Ciencias Morfol, Lab Neuroanat & Neurol Expt, E-15782 Santiago De Compostela, Spain Univ Santiago de Compostela Santiago De Compostela Spain E-15782 , Spain
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 8, volume: 31, anno: 2001,
pagine: 986 - 998
SICI:
0891-5849(20011015)31:8<986:EOIAMO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NIGROSTRIATAL DOPAMINE NEURONS; X-RAY-MICROANALYSIS; PARKINSONS-DISEASE; SUBSTANTIA-NIGRA; OXIDATIVE STRESS; IN-VITRO; INTRASTRIATAL INJECTION; LIPID-PEROXIDATION; TRANSITION-METALS; COMPLEX-I;
Keywords:
6-hydroxydopamine; hydroxyl radical; metal ions; antioxidants; dopaminergic neurotoxicity; Parkinson's disease; free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Soto-Otero, R Univ Santiago de Compostela, Fac Med, Dept Bioquim & Biol Mol, Grp Neuroquim, San Francisco 1, E-15782 Santiago De Compostela, Spain Univ Santiago de Compostela San Francisco 1 Santiago De Compostela Spain E-15782
Citazione:
E. Mendez-Alvarez et al., "Effect of iron and manganese on hydroxyl radical production by 6-hydroxydopamine: Mediation of antioxidants", FREE RAD B, 31(8), 2001, pp. 986-998

Abstract

6-Hydroxydopamine (6-OHDA) neurotoxicity has often been related to the generation of free radicals. Here we examined the effect of the presence of iron (Fe2+ and Fe3+) and manganese and the mediation of ascorbate, L-cysteine(CySH), glutathione (GSH), and N-acetyl-CySH on hydroxyl radical ((OH)-O-.) production during 6-OHDA autoxidation. In vitro, the presence of 800 nM iron increased (> 100%) the production of (OH)-O-. by 5 muM 6-OHDA while Mn2 caused a significant reduction (72%). The presence of ascorbate (100 muM)induced a continuous generation of (OH)-O-. while the presence of sulfhydryl reductants (100 muM) limited this production to the first minutes of thereaction. In general, the combined action of metal + antioxidant increasedthe (OH)-O-. production, this effect being particularly significant (> 400%) with iron + ascorbate. In vivo, tyrosine hydroxylase immunohistochemistry revealed that intrastriatal injections of rats with 6-OHDA (30 nmol) + ascorbate (600 nmol), 6-OHDA + ascorbate + Fe2+ (5 nmol), and 6-OHDA + ascorbate + Mn2+ (5 nmol) caused large striatal lesions, which were markedly reduced (60%) by the substitution of ascorbate by CySH. Injections of Fe2+ or Mn2+ alone showed no significant difference to those of saline. These results clearly demonstrate the role of ascorbate as an essential element for theneurotoxicity of 6-OHDA, as well as the diminishing action of sulfhydryl reductants, and the negliglible effect of iron and manganese on 6-OHDA neurotoxicity. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 23:11:51