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Titolo:
Production of transgenic rats using young Sprague-Dawley females treated with PMSG and hCG
Autore:
Hirabayashi, M; Ito, K; Sekimoto, A; Hochi, S; Ueda, M;
Indirizzi:
YS New Technol Inst Inc, Ishibashi, Tochigi 3290512, Japan YS New Technol Inst Inc Ishibashi Tochigi Japan 3290512 gi 3290512, Japan Shinshu Univ, Fac Text Sci & Technol, Ueda, Nagano 3868567, Japan Shinshu Univ Ueda Nagano Japan 3868567 chnol, Ueda, Nagano 3868567, Japan
Titolo Testata:
EXPERIMENTAL ANIMALS
fascicolo: 5, volume: 50, anno: 2001,
pagine: 365 - 369
SICI:
1341-1357(200110)50:5<365:POTRUY>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
EARLY EMBRYO DEVELOPMENT; MARE SERUM GONADOTROPIN; IMMATURE RATS; OVARIAN-FUNCTION; FERTILIZATION; HORMONE; SUPEROVULATION; INVITRO; EGGS;
Keywords:
microinjection; Sprague-Dawley; superovulation; synchronization; transgenic rats;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Hirabayashi, M YS New Technol Inst Inc, 519 Shimoishibashi, Ishibashi, Tochigi 3290512, Japan YS New Technol Inst Inc 519 Shimoishibashi Ishibashi Tochigi Japan 3290512
Citazione:
M. Hirabayashi et al., "Production of transgenic rats using young Sprague-Dawley females treated with PMSG and hCG", EXP ANIM, 50(5), 2001, pp. 365-369

Abstract

The aim of this study was to examine the effects of gonadotrophin treatments on estrus synchronization and superovulation in young Sprague-Dawley (SD) rats that had not yet exhibited defined estrus cycles (5 to 7 weeks old),and to produce transgenic rats using these females as embryo donors and recipients. In Experiment 1, female rats were injected with PMSG and hCG (12.5, 25, 50 and 100 IU/kg each) and were mated with stud males. The reproductive performance of young rats were highest when PMSG and hCG at doses of 25IU/kg each were injected (delivery rate 87.5%, nursing rate 92.9%). In Experiment 2, female rats were injected with PMSG and hCG (100, 150 and 300 IU/kg each) to induce superovulation. More eggs were recovered from the rats injected with PMSG and hCG at 150 and 300 IU/kg than from those treated with 100 IU/kg (33.4 and 41.3 vs. 13.3 eggs per female, respectively, p <0.05). In Experiment 3, pronuclear-stage zygotes from 150 IU/kg PMSG/hCG-treatedrats were used for microinjection of the fusion gene of bovine alpha S1-casein gene promoter and human growth hormone gene (2.8 kb), and the microinjected zygotes were transferred into the oviduct ampullae of the 25 IU/kg PMSG/hCG-treated rats. Seventeen transgenic rats were obtained from the 334 DNA-injected zygotes (5.1%). These results indicate that recipients and embryo donors for the production of transgenic rats can be prepared by the appropriate PMSG and hCG treatments of young SD rats, regardless of their estrus stages.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 11:03:57