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Titolo:
ATP stimulation of P2X(7) receptors activates three different ionic conductances on cultured mouse Schwann cells
Autore:
Colomar, A; Amedee, T;
Indirizzi:
Inst Francois Magendie, INSERM, U394, F-33077 Bordeaux, France Inst Francois Magendie Bordeaux France F-33077 F-33077 Bordeaux, France
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 6, volume: 14, anno: 2001,
pagine: 927 - 936
SICI:
0953-816X(200109)14:6<927:ASOPRA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
DORSAL-ROOT GANGLIA; MICROGLIAL CELLS; NUCLEOTIDE RECEPTOR; EXTRACELLULAR ATP; DENDRITIC CELLS; CHANNELS; RELEASE; POTASSIUM; MEMBRANE; SUBUNITS;
Keywords:
ATP; Ca2+-activated K+ current; Cl- conductance; P2X(7) receptor; Schwann cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Amedee, T Inst Francois Magendie, INSERM, U394, Rue Camille St Saens, F-33077 Bordeaux, France Inst Francois Magendie Rue Camille St Saens Bordeaux France F-33077
Citazione:
A. Colomar e T. Amedee, "ATP stimulation of P2X(7) receptors activates three different ionic conductances on cultured mouse Schwann cells", EUR J NEURO, 14(6), 2001, pp. 927-936

Abstract

Extracellular ATP, by acting on P2 purinergic receptors, is a potent mediator of cell-to-cell communication both within and between the nervous and the immune systems. We show here by patch-clamp recording, fluorescent dye uptake and immunocytochemistry that, in cultured mouse Schwann cells, ATP activates a P2X(7) receptor associated with three different ionic conductances. In control conditions, ATP activated an inward current (I-ATP) with a low potency (EC50, 7.2 mM). Replacing ATP either by the ATP analogue 2',3'-O-(4-benzoyl-4-benzoyl)-ATP (BzATP) or by the tetraacidic form ATP(4-) potentiated the inward current (ATP(4-) EC50, 375 muM). ATP and BzATP currents were strongly reduced by periodate oxidized ATP (oATP), an antagonist of P2X(7) receptors. I-ATP was a mixed current composed of a nonselective cationicconductance, a cationic conductance selective for K+ and an anionic conductance selective for Cl-. The activation of the K+ conductance was dependenton an influx of Ca2+, and was blocked by charybdotoxin (ChTX) and tetraethylammonium (TEA), two potent antagonists of large conductance Ca2+- activated K+ channels (BK channels). The activation of the Cl- conductance was insensitive to Ca2+ but required the presence of K+. Total removal of K+ blocked both the Ca2+-activated K+ conductance and the Cl- conductance, unveiling the P2X(7) nonselective cationic conductance. The P2X(7) receptor was localized by immunocytochemistry using a polyclonal antibody, anti-P2X(7), whilst its expression and functionality were both detected by the uptake of Lucifer Yellow. This receptor could regulate the synthesis and the release ofcytokines by Schwann cells during pathophysiological events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:26:45