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Titolo:
Independent receptors for diadenosine pentaphosphate and ATP in rat midbrain single synaptic terminals
Autore:
Diaz-Hernandez, M; Pintor, J; Castro, E; Miras-Portugal, MT;
Indirizzi:
Univ Complutense Madrid, Fac Vet, Dept Bioquim & Biol Mol 4, E-28040 Madrid, Spain Univ Complutense Madrid Madrid Spain E-28040 ol 4, E-28040 Madrid, Spain
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 6, volume: 14, anno: 2001,
pagine: 918 - 926
SICI:
0953-816X(200109)14:6<918:IRFDPA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GATED ION CHANNELS; GUINEA-PIG BRAIN; NERVOUS-SYSTEM; P2X RECEPTORS; DIINOSINE PENTAPHOSPHATE; POLYPHOSPHATES; SYNAPTOSOMES; NEURONS; TRANSMISSION; SELECTIVITY;
Keywords:
ATP; calcium voltage channels; diadenosine polyphosphates; microfluorimetry; synaptosomes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Miras-Portugal, MT Univ Complutense Madrid, Fac Vet, Dept Bioquim & Biol Mol 4, E-28040 Madrid, Spain Univ Complutense Madrid Madrid Spain E-28040 d, Spain
Citazione:
M. Diaz-Hernandez et al., "Independent receptors for diadenosine pentaphosphate and ATP in rat midbrain single synaptic terminals", EUR J NEURO, 14(6), 2001, pp. 918-926

Abstract

Diadenosine pentaphosphate (ApsA) and adenosine 5'-triphosphate (ATP) stimulate a intrasynaptosomal calcium, concentration [Ca2+](i) increase via specific purinergic receptors, in; rat midbrain synaptosomes, although nothingis known: about their distribution in presynaptic terminals. A microfluorimetric technique to measure [Ca2+](i) increase using the dye FURA-2AM,, haspermitted study of the presence of dinucleotide and P2X receptors in independent isolated synaptic terminals. Our results demonstrate the existence of three populations of synaptosomes: one with dinucleotide receptors (12%),another with P2X receptors (20%) and a third with both (14%). It has been possible to demonstrate that the activation of these receptors occurs only in the presence of extracellular Ca2+ and that it is also coupled with voltage-dependent Ca2+ channels. Finally 54% of the synaptosomes that respondedto K+ did not present any calcium increase mediated by the nucleotides used. In summary, ATP and dinucleotides exhibit specific ionotropic receptors,that can coexist or not on the same synaptic terminal.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:10:52