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Titolo:
Capsaicin-evoked CGRP release from rat buccal mucosa: development of a model system for studying trigeminal mechanisms of neurogenic inflammation
Autore:
Flores, CM; Leong, AS; Dussor, GO; Harding-Rose, C; Hargreaves, KM; Kilo, S;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Endodont, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 t Endodont, San Antonio, TX 78229 USA Univ Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 Pharmacol, San Antonio, TX 78229 USA Univ Minnesota, Dept Restorat Sci, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 at Sci, Minneapolis, MN 55455 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 14, anno: 2001,
pagine: 1113 - 1120
SICI:
0953-816X(200110)14:7<1113:CCRFRB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-RELATED-PEPTIDE; PLASMA-PROTEIN EXTRAVASATION; SUBSTANCE-P; ORAL-MUCOSA; NEUROCHEMICAL MARKERS; VASCULAR-PERMEABILITY; JUNCTIONAL EPITHELIUM; NERVE-FIBERS; DENTAL-PULP; CALCITONIN;
Keywords:
exocytosis; in vitro superfusion; neuropeptide; nociceptor; pain; sensory neuron;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Flores, CM Univ Texas, Hlth Sci Ctr, Dept Endodont, 7703 Floyd Curl Dr, San Antonio, TX 78229 USA Univ Texas 7703 Floyd Curl Dr San Antonio TX USA 78229 8229 USA
Citazione:
C.M. Flores et al., "Capsaicin-evoked CGRP release from rat buccal mucosa: development of a model system for studying trigeminal mechanisms of neurogenic inflammation", EUR J NEURO, 14(7), 2001, pp. 1113-1120

Abstract

Many of the physiological hallmarks associated with neurogenic inflammatory processes in cutaneous tissues are similarly present within orofacial structures. Such attributes include the dependence upon capsalcin-sensitive sensory neurons and the involvement of certain inflammatory mediators derivedtherein, including calcitonin gene-related peptide (CGRP). However, there are also important differences between the trigeminal and spinal nervous systems, and the potential contributions of neurogenic processes to inflammatory disease within the trigeminal system have yet to be fully elucidated. We present here a model system that affords the ability to study mechanisms regulating the efferent functions of peptidergic terminals that may subserve neurogenic inflammation within the oral cavity. Freshly dissected buccal mucosa tissue from adult, male, Sprague-Dawley rats was placed into chambers and superfused with oxygenated, Krebs buffer. Serial aliquots of the egressing superfusate were acquired and analysed by radioimmunoassay for immunoreactive CGRP (iCGRP). Addition of the selective excitotoxin, capsaicin (10-300 muM), to the superfusion buffer resulted in a significant, concentration-dependent increase in superfusater levels of iCGRP. Similarly, release of iCGRP from the buccal mucosa could also be evoked by a depolarizing concentration of potassium chloride (50 mm) or by the calcium ionophore A23187 (1 muM). The specific, capsaicin receptor antagonist, capsazepine (300 muM),completely abolished the capsaicin-evoked release of ICGRP while having noeffect whatsoever on the potassium-evoked release. Moreover, capsaicin-evoked release was dependent upon the presence of extracellular calcium ions and was significantly, though incompletely, attenuated by neonatal capsaicindenervation. Collectively, these data indicate that the evoked neurosecretion of iCGRP in response to capsaicin occurs via a vanilloid receptor-mediated, exocytotic mechanism. The model system described here should greatly facilitate future investigations designed to identify and characterize the stimuli that regulate the release of CGRP or other neurosecretory substancesin isolated tissues. This system may also be used to elucidate the role ofthese mediators in the aetiology of inflammatory processes within the trigeminal field of innervation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:42:29