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Titolo:
Subunit composition of strychnine-sensitive glycine receptors expressed byadult rat basolateral amygdala neurons
Autore:
McCool, BA; Farroni, JS;
Indirizzi:
Texas A&M Univ Syst, Hlth Sci Ctr, Dept Med Pharmacol & Toxicol, College Stn, TX 77843 USA Texas A&M Univ Syst College Stn TX USA 77843 l, College Stn, TX 77843 USA
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 7, volume: 14, anno: 2001,
pagine: 1082 - 1090
SICI:
0953-816X(200110)14:7<1082:SCOSGR>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-AMINOBUTYRIC-ACID; BETA-SUBUNIT; SPINAL-CORD; CHROMOSOMAL LOCALIZATION; FUNCTIONAL-CHARACTERIZATION; CHOLINERGIC INTERNEURONS; CEREBRAL-CORTEX; BINDING SUBUNIT; MESSENGER-RNAS; GLIAL-CELLS;
Keywords:
heterologous expression; limbic forebrain; picrotoxin; single-cell RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: McCool, BA Texas A&M Univ Syst, Hlth Sci Ctr, Dept Med Pharmacol & Toxicol, 368 Reynolds Med Bldg,Mail Stop 1114, College Stn, TX 77843 USA Texas A&MUniv Syst 368 Reynolds Med Bldg,Mail Stop 1114 College Stn TX USA 77843
Citazione:
B.A. McCool e J.S. Farroni, "Subunit composition of strychnine-sensitive glycine receptors expressed byadult rat basolateral amygdala neurons", EUR J NEURO, 14(7), 2001, pp. 1082-1090

Abstract

In neonatal rats, strychnine-sensitive glycine receptors are widely expressed in the spinal cord, brainstem and forebrain. During development, these 'neonatal' receptors are replaced by an adult isoform, the expression of which becomes restricted primarily to brain stem and spinal cord. Unlike mostforebrain regions, functional strychnine-sensitive glycine receptors appear to persist within adult rat amygdala. However, the subunit composition ofglycine receptors expressed by amygdala neurons and its relationship to the adult isoform in brain stem/spinal cord has not been defined precisely. In this report, we have utilized RT-PCR and single-cell RT-PCR to demonstrate that the 'neonatal' alpha (2)-subunit mRNA persists in adult rat amygdalaneurons and is the predominant (alpha -subunit. We further demonstrate that native amygdala glycine receptors are relatively insensitive to the receptor antagonist picrotoxin, suggesting that alpha (2)- and beta -subunits may be present together in the same multisubunit complex. We further demonstrate that alpha (2)- and beta -subunits cloned from adult rat amygdala can form functional channels when expressed in a heterologous system. Together, these studies highlight both the unique characteristics of strychnine-sensitive glycine receptors in the adult rat amygdala as well as the possibilitythat beta (2)/beta channels may represent the adult forebrain isoform of the strychnine-sensitive glycine receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:58:18