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Titolo:
Usefulness of adding multiplex nested-polymerase chain reaction assay of cerebrospinal fluid samples to routine diagnostic testing for herpesvirus encephalitis
Autore:
Frias, C; Matas, L; Ferre, X; Millan, M; Marti, S; Hernandez, A; Ausina, V;
Indirizzi:
Univ Badalona, Hosp Germans Trias & Pujol, Dept Microbiol, Badalona 08916,Spain Univ Badalona Badalona Spain 08916 Dept Microbiol, Badalona 08916,Spain Univ Badalona, Hosp Germans Trias & Pujol, Dept Neurol, Badalona 08916, Spain Univ Badalona Badalona Spain 08916 l, Dept Neurol, Badalona 08916, Spain Univ Badalona, Hosp Germans Trias & Pujol, Dept Internal Med, Badalona 08916, Spain Univ Badalona Badalona Spain 08916 t Internal Med, Badalona 08916, Spain
Titolo Testata:
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
fascicolo: 9, volume: 20, anno: 2001,
pagine: 670 - 672
SICI:
0934-9723(200109)20:9<670:UOAMNC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; SIMPLEX VIRUS-INFECTIONS; DNA; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Frias, C Univ Badalona, Hosp Germans Trias & Pujol, Dept Microbiol, Carretera Canyet S-N, Badalona 08916, Spain Univ Badalona Carretera Canyet S-N Badalona Spain 08916 6, Spain
Citazione:
C. Frias et al., "Usefulness of adding multiplex nested-polymerase chain reaction assay of cerebrospinal fluid samples to routine diagnostic testing for herpesvirus encephalitis", EUR J CL M, 20(9), 2001, pp. 670-672

Abstract

The present study was conducted to assess the usefulness of adding the multiplex nested-polymerase chain reaction assay of cerebrospinal fluid samples to routine diagnostic testing for herpesvirus encephalitis and to monitorthe efficacy of therapy. Cerebrospinal fluid samples from 45 patients withpresumed herpesvirus encephalitis were tested for herpes simplex virus, varicella-zoster virus, cytomegalovirus, human herpesvirus 6, and Epstein-Barr virus. Ten of the 45 patients were positive for a virus using the polymerase chain reaction assay: herpes simplex virus (n = 5), Epstein-Barr virus (n = 3). and herpes simplex virus plus the Epstein-Barr virus (n = 2). Cerebrospinal fluid from two patients who had undergone acyclovir therapy gave negative results. Analysis of cerebrospinal fluid by multiplex polymerase chain reaction can be useful for establishing an accurate diagnosis and as amarker of the efficacy of therapy.

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Documento generato il 26/11/20 alle ore 14:24:31