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Titolo:
Efficacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs
Autore:
Wirtanen, G; Aalto, M; Harkonen, P; Gilbert, P; Mattila-Sandholm, T;
Indirizzi:
VTT Biotechnol, Espoo, Finland VTT Biotechnol Espoo FinlandVTT Biotechnol, Espoo, Finland Univ Manchester, Manchester, Lancs, England Univ Manchester Manchester Lancs England ter, Manchester, Lancs, England
Titolo Testata:
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
fascicolo: 4-5, volume: 213, anno: 2001,
pagine: 409 - 414
SICI:
1438-2377(200110)213:4-5<409:ETOCDA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TEST TUBE; BACTERIAL;
Keywords:
disinfection; biofilm; hygiene; efficacy testing; poloxamer; hydrogels;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Wirtanen, G VTT Biotechnol, Tietotie 2, Espoo, Finland VTT Biotechnol Tietotie 2 Espoo Finland ie 2, Espoo, Finland
Citazione:
G. Wirtanen et al., "Efficacy testing of commercial disinfectants against foodborne pathogenic and spoilage microbes in biofilm-constructs", EUR FOOD RE, 213(4-5), 2001, pp. 409-414

Abstract

This paper describes the evaluation of poloxamer-hydrogel biofilm-constructs for the routine efficacy testing of disinfectants at normal use strength. Aqueous solutions of poloxamer Pluronic F127 show thermoreversible gelation, being liquid at temperatures <15 <degrees>C but firm gels at temperatures >15 degreesC. Chilled poloxamer solutions (30% w/v) were made up in a tryptone soy broth and inoculated with stationary-phase cultures of 14 foodborne spoilage microbes, including Pseudomonas, Bacillus, Staphylococcus, Micrococcus, enterobacteria and a yeast, as well as pathogen test-strains, including Listeria and Salmonella. Drops (either 200 mul or 100 mul) were placed onto pre-warmed, sterile, stainless steel discs held in sealed Petri dishes. The constructs were incubated for 5 h at 30 degreesC and all strains grew well in the poloxamer hydrogel. Incubated poloxamer gels and their discs were transferred to solutions of commercial disinfectant formulations containing either amphoteric surfactants, hydrogen peroxide with peracetic acid or silver ions, sodium hypochlorite, or alcohols with and without additives. After 5 min at 25 degreesC the test pieces were removed from the disinfectant solution and transferred to a neutraliser at 10-15 degreesC. These tests were carried out in triplicate. The gets dispersed rapidly, releasing the cells and enabling a count of the viable cells. All formulations effected a >5-log kill of planktonic challenges within 5 min. An effective killing of microbial cells within the biofilm-constructs was shown when the reduction was at least 0.3 log units. The results were highly reproducible, withpatterns of susceptibility varying as a function of the organism, disinfectant type, and concentration. The experiments support the view that poloxamer hydrogels can be used for testing the disinfectant efficacy of various formulations against contaminants isolated from food and drink processes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 13:30:43