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Titolo:
Identification and characterisation of beta-adrenoceptors on intact equineperipheral blood lymphocytes with the radioligand (-)-[I-125]-iodocyanopindolol
Autore:
Abraham, G; Brodde, OE; Ungemach, FR;
Indirizzi:
Univ Leipzig, Inst Pharmacol Pharm & Toxicol, Fac Med Vet, D-7010 Leipzig,Germany Univ Leipzig Leipzig Germany D-7010 Fac Med Vet, D-7010 Leipzig,Germany Univ Halle Wittenberg, Inst Pharmacol & Toxicol, Halle Saale, Germany UnivHalle Wittenberg Halle Saale Germany Toxicol, Halle Saale, Germany
Titolo Testata:
EQUINE VETERINARY JOURNAL
fascicolo: 5, volume: 33, anno: 2001,
pagine: 487 - 493
SICI:
0425-1644(200109)33:5<487:IACOBO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENERGIC-RECEPTORS; MONONUCLEAR LEUKOCYTES; BINDING; DENSITY; CELLS; BETA-2-ADRENOCEPTORS; RESPONSIVENESS; CGP-12177; LIGAND; ASTHMA;
Keywords:
horse; beta-adrenoceptors; (-)-[I-125]-iodocyanopindolol; peripheral blood lymphocytes; cyclic AMP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Ungemach, FR Univ Leipzig, Inst Pharmacol Pharm & Toxicol, Fac Med Vet, D-7010 Leipzig,Germany Univ Leipzig Leipzig Germany D-7010 D-7010 Leipzig,Germany
Citazione:
G. Abraham et al., "Identification and characterisation of beta-adrenoceptors on intact equineperipheral blood lymphocytes with the radioligand (-)-[I-125]-iodocyanopindolol", EQUINE V J, 33(5), 2001, pp. 487-493

Abstract

In this study, beta -adrenoceptors of intact equine lymphocytes were identified and subclassified by (-)-[I-125]-iodocyanopindolol (ICYP) binding. ICYP binding to intact equine lymphocytes was rapid, saturable (maximal number of binding sites 320 +/- 20 ICYP binding sites/cell, n = 12) and of high affinity (K-D value for ICYP 14.4 +/- 1.7 pmol/l, n = 12). Binding was stereospecific as shown by the 10 times greater potency of (-)-propranolol to inhibit binding than its (+)-isomer. beta -adrenoceptor agonists inhibited ICYP binding with an order of potency: (-)-isoprenaline >(-)-adrenaline >(-)-noradrenaline; the same order of potency was obtained for agonist-induced stimulation of lymphocyte cyclic AMP content. The selective beta (2)-adrenoceptor antagonist ICI 118,551 was about 1000 times more potent in inhibiting ICYP binding than was the beta (1)-selective adrenoceptor antagonist CGP 20712A. It is, therefore, concluded that in intact equine lymphocytes, ICYP labels a class of functional beta -adrenoceptors that belong predominantly (> 90%) to the beta (2)-adrenoceptor subtype; a small (< 10%) beta (1)-adrenoceptor component, however, cannot be ruledout completely. ICYP binding to equine lymphocytes might be a suitable model to study function and regulation of the beta -adrenoceptor system in thehorse in vivo. The aim of this study was to characterise the P-adrenoreceptor subtypes present on equine lymphocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:44:02