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Titolo:
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing
Autore:
Kadener, S; Cramer, P; Nogues, G; Cazalla, D; de la Mata, M; Fededa, JP; Werbajh, SE; Srebrow, A; Kornblihtt, AR;
Indirizzi:
Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Ciencias Biol, Lab Fisiol & Biol Mol, Buenos Aires, DF, Argentina Univ Buenos Aires Buenos AiresDF Argentina Buenos Aires, DF, Argentina
Titolo Testata:
EMBO JOURNAL
fascicolo: 20, volume: 20, anno: 2001,
pagine: 5759 - 5768
SICI:
0261-4189(20011015)20:20<5759:AEOTAV>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; HUMAN FIBRONECTIN GENE; POLYMERASE-II; ACTIVATION DOMAINS; SR-PROTEINS; TRANSCRIPTION; REPLICATION; ENHANCER; PROMOTER; DNA;
Keywords:
alternative splicing; SV40 T-Ag; VP16;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kornblihtt, AR Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Ciencias Biol, Lab Fisiol & Biol Mol, Pabellon 2,C1428EHA, Buenos Aires, DF, Argentina Univ Buenos Aires Pabellon 2,C1428EHA Buenos Aires DF Argentina
Citazione:
S. Kadener et al., "Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing", EMBO J, 20(20), 2001, pp. 5759-5768

Abstract

Here we investigate the promoter control of alternative splicing by studying two transcriptional activators on templates under replicating conditions. SV40 large T-antigen (T-Ag) activates template replication only 2-fold but transcription 25-fold. T-Ag-mediated replication, reported to inhibit RNApolymerase II elongation, provokes a 10- to 30-fold increase in the inclusion of the fibronectin EDI exon into mature mRNA. The T-Ag effect is exon specific, occurs in cis and depends strictly on DNA replication and not on cell transformation. VP16, an activator of transcriptional initiation and elongation, has a similar effect on transcription but the opposite effect on splicing: EDI inclusion is inhibited by 35-fold. VP16 completely reverts the T-Ag effect, but a VP16 mutant with reduced elongation ability provokes only partial reversion. Both T-Ag and VP16 promote conspicuous co-localization of mRNA with nuclear speckles that contain the SR protein SF2/ASF, a positive regulator of EDI inclusion. Therefore, we conclude that co-localization of transcripts and speckles is not sufficient to stimulate EDI inclusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:18:43