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Titolo:
Genetic polymorphisms of the human P2X(7) receptor and relationship to function
Autore:
Wiley, JS; Gu, BJ; Zhang, WY; Worthington, RA; Dao-Ung, P; Shemon, AN; Sluyter, R; Liang, S; Barden, JA;
Indirizzi:
Univ Sydney, Dept Med, Sydney, NSW 2006, Australia Univ Sydney Sydney NSWAustralia 2006 pt Med, Sydney, NSW 2006, Australia Univ Sydney, Dept Anat & Histol, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 Histol, Sydney, NSW 2006, Australia
Titolo Testata:
DRUG DEVELOPMENT RESEARCH
fascicolo: 2-3, volume: 53, anno: 2001,
pagine: 72 - 76
SICI:
0272-4391(200106/07)53:2-3<72:GPOTHP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR ATP; DENDRITIC CELLS; ION CHANNELS; ACTIVATION; APOPTOSIS; LYMPHOCYTES; EXPRESSION; INDUCTION; DEATH;
Keywords:
P2X(7) receptor; non-functional P2X(7); polymorphisms of P2X(7); loss-of-function mutation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Wiley, JS Nepean Hosp, Clin Sci Bldg, Penrith, NSW 2750, Australia Nepean Hosp Clin Sci Bldg Penrith NSW Australia 2750 , Australia
Citazione:
J.S. Wiley et al., "Genetic polymorphisms of the human P2X(7) receptor and relationship to function", DRUG DEV R, 53(2-3), 2001, pp. 72-76

Abstract

Extracellular ATP has been shown to induce apoptotic death of many cell types of hematopoietic origin. This action of ATP is mediated via activation of P2X(7) purinergic receptors, which show the unusual property of time-dependent channel dilation to accept permeants as large as ethidium cation (314 Da). P2X(7) function, measured by area under the ATP-induced ethidium uptake curve, was 5-fold greater for monocytes than lymphocytes, while polymorphs and platelets showed no ethidium uptake. Expression of P2X(7) receptor,measured by the binding of a monoclonal antibody, was also 5-fold greater on monocytes than lymphocytes. However, in some subjects, both normal and with chronic lymphocytic leukemia, the P2X(7) receptor was nonfunctional despite good expression of P2X7 protein. Three single nucleotide polymorphismswere found in the P2X(7) cDNA coding region, one of which correlated with P2X(7) function. Thus, the homozygous substitution of alanine for glutamic acid at amino acid 496 led to complete loss of function of the P2X(7) receptor, while the heterozygous polymorphism gave function half that of the germline P2X(7) receptor. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:05:42