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Titolo:
Determination of sulfur-containing antibiotics using high-performance liquid chromatography with integrated pulsed amperometric detection
Autore:
Hanko, VP; LaCourse, WR; Dasenbrock, CO; Rohrer, JS;
Indirizzi:
Dionex Chem Corp, Sunnyvale, CA 94088 USA Dionex Chem Corp Sunnyvale CA USA 94088 hem Corp, Sunnyvale, CA 94088 USA Univ Maryland Baltimore Cty, Dept Chem & Biochem, Baltimore, MD 21228 USA Univ Maryland Baltimore Cty Baltimore MD USA 21228 altimore, MD 21228 USA
Titolo Testata:
DRUG DEVELOPMENT RESEARCH
fascicolo: 4, volume: 53, anno: 2001,
pagine: 268 - 280
SICI:
0272-4391(200108)53:4<268:DOSAUH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROCHEMICAL DETECTION; ION CHROMATOGRAPHY; GOLD ELECTRODES; AMINO-ACIDS; WAVE-FORM; HPLC; HOMOCYSTEINE; METHIONINE; RESISTANCE; SULFIDE;
Keywords:
antibiotics, sulfur-containing; electrochemical detection; integrated pulsed amperometric detection; amperometry; PAD; IPAD; high-performance liquid chromatography; HPLC; stability; purity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Hanko, VP Dionex Chem Corp, 500 Mercury Dr, Sunnyvale, CA 94088 USA DionexChem Corp 500 Mercury Dr Sunnyvale CA USA 94088 94088 USA
Citazione:
V.P. Hanko et al., "Determination of sulfur-containing antibiotics using high-performance liquid chromatography with integrated pulsed amperometric detection", DRUG DEV R, 53(4), 2001, pp. 268-280

Abstract

Sulfur-containing antibiotics (e.g., ampicillin, penicillin, lincomycin, cephalosporin, and sulfanilamide) are a general class of compounds that haveantibacterial properties. Some of these antibiotics lack a strong chromophore, and consequently are poorly detected using absorbance detection. The presence of oxidizable sulfur within an antibiotic's molecular structure allows its direct detection by pulsed amperometry. Pulsed amperometry is an electrochemical detection method that uses a pulsed potential-time waveform at a noble metal electrode surface to electrocatalytically oxidize analytes in proportion to concentration. The current for sulfur compounds on a gold (Au) electrode is caused by the desorption of the analyte previously adsorbed at the oxide-free Au surface. Current integration in conjunction with rapidly changed voltage potentials is termed integrated pulsed amperometric detection (IPAD). Recent publications using high-performance liquid chromatography (HPLC) with IPAD have demonstrated the capability to measure sulfur antibiotics. Our investigations of HPLC-IPAD showed poor long-term peak area reproducibility for these drugs. In this article, we present a new IPAD waveform that we demonstrate has good long-term (>2 months) peak area reproducibility (4-5% relative s.d.). We show that a broad spectrum of sulfur-antibiotic chemical structures can be detected, and we compare IPAD to absorbance detection. For nonchromophoric sulfur-containing antibiotics, IPAD showed greater sensitivity than absorbance detection. The lower limits of detection, linearities, and peak area precision are compared for both [PAD and absorbance detection methods using seven antibiotics representing a diversity of chemical structures, including one nonsulfur-containing antibiotic. The stabilities of cephradine and cephapirin were monitored using both IPAD and absorbance detections. Cephradine showed high chemical stability in aqueous ambient conditions at 10 mug/ml, whereas cephapirin showed a decomposition rate of 10% per day by both detection techniques. Some decomposition products could only be detected by IPAD. High recoveries were demonstrated for two antibiotics (sulfamethoxazole and trimethoprim) in a pharmaceutical tablet formulation. For the same formulation, the feasibility of conducting a dissolution study was also demonstrated. IPAD is a good primary detectionmethod to complement or replace absorbance detection for nonchromophoric sulfur-containing antibiotics and their sulfur-containing impurities and decomposition products. HPLC-IPAD is also a good confirming technique for sulfur antibiotics with chromophoric properties, and their impurities and decomposition products. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 04/04/20 alle ore 02:30:20