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Titolo:
K+ conductance activated during regulatory volume decrease. The channels in Ehrlich cells and their possible molecular counterpart
Autore:
Niemeyer, MI; Cid, LP; Sepulveda, FV;
Indirizzi:
Ctr Estudios Cientif, Valdivia, Chile Ctr Estudios Cientif Valdivia Chile r Estudios Cientif, Valdivia, Chile Univ Chile, Fac Med, ICBM, Santiago, Chile Univ Chile Santiago ChileUniv Chile, Fac Med, ICBM, Santiago, Chile Univ Chile, Fac Med, ICBM, Santiago, Chile Univ Chile Santiago ChileUniv Chile, Fac Med, ICBM, Santiago, Chile
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 565 - 575
SICI:
1095-6433(200110)130:3<565:KCADRV>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASCITES TUMOR-CELLS; RECTIFIER POTASSIUM CHANNEL; MEMBRANE MECHANISMS; ARACHIDONIC-ACID; TAURINE EFFLUX; DISTAL COLON; FATTY-ACIDS; CURRENTS; BLOCK; PH;
Keywords:
Ehrlich cells; regulatory volume decrease; potassium channel; mTASK-2; patch-clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Niemeyer, MI Ctr Estudios Cientif, Valdivia, Chile Ctr Estudios Cientif Valdivia Chile entif, Valdivia, Chile
Citazione:
M.I. Niemeyer et al., "K+ conductance activated during regulatory volume decrease. The channels in Ehrlich cells and their possible molecular counterpart", COMP BIOC A, 130(3), 2001, pp. 565-575

Abstract

K+ currents activated by hypotonic cell swelling have been studied in Ehrlich ascites tumour cells by the whole-cell recording mode of the patch-clamp technique. K+ together with Cl- currents developed in the absence of added intracellular Ca2+ and with strong buffering of internal Ca2+ in experiments conducted at 37 degreesC. Manipulation of the extracellular medium withother cations suggests a selectivity sequence of K+ > Rb+ > NH4+ greater than or equal to Na+ congruent to Li+ congruent to Cs+. The current-voltage relationship of the volume-sensitive K+ current was well fitted with the Goldman-Hodgkin-Katz current equation between - 130 and 20 mV at both physiological and high K+ extracellular solutions. The class III antiarrhytmic drug clofilium blocked the volume-sensitive K+ current in a voltage-independent manner. Clofilium was also found to be a strong inhibitor of the regulatory volume decrease (RVD) response of Ehrlich cells. The leukotriene D-4 (LTD4) can activate the same current in isotonicity, consistent with a role for this compound in the signalling process of volume regulation. It is suggested that K+ channels activated by cell swelling belong to the so-called background K+ channel group. These are voltage-independent channels which underlie the resting potential of many cells and have recently been identifiedas belonging to a family of K+ channels with two pore domains in tandem (2P-4TM). Preliminary experiments show the presence of the TASK-2 channel, a member of the 2P-4TM family inhibited by acid extracellular pH, in Ehrlich cells and suggest that it might underlie the swelling-induced K+ current. (C) 2001 Elsevier Science Inc. All rights reserved.

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Documento generato il 09/04/20 alle ore 07:38:20