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Titolo:
Expression of cystic fibrosis transmembrane conductance regulator in the skin of the toad, Bufo bufo and possible role for Cl- transport across the heterocellular epithelium
Autore:
Amstrup, J; Froslev, J; Willumsen, NJ; Mobjerg, N; Jespersen, A; Larsen, EH;
Indirizzi:
Univ Copenhagen, August Krogh Inst, Zoophysiol Lab, DK-2100 Copenhagen O, Denmark Univ Copenhagen Copenhagen Denmark O Lab, DK-2100 Copenhagen O, Denmark Univ Copenhagen, Inst Zool, Dept Zoomorphol, Copenhagen 0, Denmark Univ Copenhagen Copenhagen Denmark 0 t Zoomorphol, Copenhagen 0, Denmark
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 539 - 550
SICI:
1095-6433(200110)130:3<539:EOCFTC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIA-RICH CELLS; ANION SELECTIVITY; CHLORIDE TRANSPORT; APICAL MEMBRANE; RANA-ESCULENTA; XENOPUS-LAEVIS; ION-TRANSPORT; CYCLIC-AMP; IDENTIFICATION; CHANNELS;
Keywords:
beta-adrenergic receptors; CFTR; chloride channels; collecting duct; cyclic AMP; intercalated cells; isoproterenol; patch clamp;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Larsen, EH Univ Copenhagen, August Krogh Inst, Zoophysiol Lab, Univ Pk 13,DK-2100 Copenhagen O, Denmark Univ Copenhagen Univ Pk 13 Copenhagen Denmark O gen O, Denmark
Citazione:
J. Amstrup et al., "Expression of cystic fibrosis transmembrane conductance regulator in the skin of the toad, Bufo bufo and possible role for Cl- transport across the heterocellular epithelium", COMP BIOC A, 130(3), 2001, pp. 539-550

Abstract

Evidence is discussed that apical CFTR Cl- channels of mitochondria-rich (MR) cells of Bufo bufo skin conduct beta -adrenergic receptor-activated Cl-currents. Ussing chambers studies revealed the following selectivity sequence of the receptor activated conductance, Cl- > Br- > NO3- > I-. With ion selective microelectrode-techniques, it was shown that receptor-coupled Cl-channels are not located in principal cells. A small conductance (7-10 pS)CFTR-like Cl- channel is located in the apical plasma membrane of MR cells. Short life times of sealed patches prevented detailed study of its selectivity to other halide ions and its molecular regulation. With monoclonal hCFTR-antibodies, selective expression in MR cells of the targeted antigens could be demonstrated. A transcript of CFTR was amplified in the skin, and abbCFTR cDNA clone was generated from toad skin mRNA that exhibits 89% amino acid identity with the human homologue. The frequency of obtaining channels in patch clamp studies was too low for accounting quantitatively for themacroscopic conductance. Since MR cells were isolated by trypsin, and a putative extracellular loop of the deduced bbCFTR protein contains a target peptide bond for trypsin, enzyme treatment may have destroyed apical CFTR molecules. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:34:42