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Titolo:
Some ways of looking at compensatory kosmotropes and different water environments
Autore:
Lever, M; Blunt, JW; Maclagan, RGAR;
Indirizzi:
Canterbury Hlth Labs, Biochem Unit, Christchurch, New Zealand Canterbury Hlth Labs Christchurch New Zealand Christchurch, New Zealand Univ Canterbury, Dept Chem, Christchurch 1, New Zealand Univ Canterbury Christchurch New Zealand 1 , Christchurch 1, New Zealand
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 471 - 486
SICI:
1095-6433(200110)130:3<471:SWOLAC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRIMETHYLAMINE-N-OXIDE; MOLECULAR-DYNAMICS SIMULATION; AB-INITIO; LACTATE-DEHYDROGENASE; AQUEOUS-SOLUTION; POTENTIAL FUNCTIONS; PROTEIN STABILITY; MODIFIED BETAINES; OSMOTIC-STRESS; UREA;
Keywords:
protein stabilization; kosmotrope; osmolyte; urea; compatible solute; near infra-red spectroscopy; surface hydration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Lever, M Canterbury Hlth Labs, Biochem Unit, Private Bag 151, Christchurch, New Zealand Canterbury Hlth Labs Private Bag 151 Christchurch New Zealand d
Citazione:
M. Lever et al., "Some ways of looking at compensatory kosmotropes and different water environments", COMP BIOC A, 130(3), 2001, pp. 471-486

Abstract

Hydration of macromolecular structures determines biological activity. Stabilizing solutes are kosmotropic (increase order of water) rather than chaotropic (decrease order). Preferential hydration of surfaces is a thermodynamic consequence of the solution behavior of kosmotropic solutes, but inconsistencies imply interactions such as the hydration of specific sites withinmacromolecules. Thermodynamic measures require bulk pure solutes; here simpler measures of the effects on bulk water, water at surfaces and hydrationwater of probes have been applied to solutes including natural stabilizers, analogues and example chaotropes. Changes in the near-infrared spectra, water proton NMR chemical shifts and relaxation times measure changes in thebulk liquid; HPLC-column retention of solutes indicate interactions with hydration water at different surfaces, and fluorescence probes detect effects on functional group hydration water. Ab initio calculations and Monte-Carlo simulations of the solutes in water measure the energetics of the solute-water interactions, the dipole moments of these molecules, their charge distributions and the effect of the solute molecules on the structure of water. The rankings of the test solutes by these measures are not consistent. Thus, stabilizing solutes are not interchangeable in biological systems and the intracellular replacement of one by another could affect the integration of cell metabolism. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:31:42