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Titolo:
Cell cycle delay and apoptosis in response to osmotic stress
Autore:
Dmitrieva, NI; Michea, LF; Rocha, GM; Burg, MB;
Indirizzi:
NHLBI, Kidney & Electrolyte Metab Lab, Bethesda, MD 20892 USA NHLBI Bethesda MD USA 20892 Electrolyte Metab Lab, Bethesda, MD 20892 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 3, volume: 130, anno: 2001,
pagine: 411 - 420
SICI:
1095-6433(200110)130:3<411:CCDAAI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE EXCISION-REPAIR; HIGH UREA CONCENTRATIONS; RENAL MEDULLARY CELLS; C-TERMINAL DOMAIN; MDCK CELLS; ORGANIC OSMOLYTES; HUMAN FIBROBLASTS; EPITHELIAL-CELLS; P53 MODULATION; PROTEIN-KINASE;
Keywords:
osmolality; renal cells; NaCl; urea; G2 arrest; cell growth; apoptosis; p53;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Burg, MB NHLBI, Kidney & Electrolyte Metab Lab, Bldg 10,Room 6N260, Bethesda, MD 20892 USA NHLBI Bldg 10,Room 6N260 Bethesda MD USA 20892 esda, MD 20892 USA
Citazione:
N.I. Dmitrieva et al., "Cell cycle delay and apoptosis in response to osmotic stress", COMP BIOC A, 130(3), 2001, pp. 411-420

Abstract

As part of the urinary concentrating mechanism, renal inner medulla cells may be exposed to extremely variable NaCl and urea concentrations that can reach very high levels. A number of studies, reviewed herein, aim to understand how such osmotic stress affects the cells and what protective mechanisms might exist. The majority of these studies are done on inner medullary epithelial cells that grow continuously in tissue culture (mIMCD3). Cells grown at 300 mosmol/kg survive increase to 500 mosmol/ka by adding NaCl or urea, but only after a growth arrest of similar to 24 h. At a higher osmolality (650-700 mosmol/kg) most cells die within hours by apoptosis. The cells both in vitro and in vivo adapt to high osmolality by a number of mechanisms, including accumulation of variety of organic osmolytes and induction of heat shock proteins. The cell cycle delay results from blocks at the G1 andG2/M checkpoints and slowing during S. After adding NaCl, but not urea, the amount and transcriptional activity of p53 (the tumor suppressor protein)increases. The p53 is phosphorylated on ser-15 and is transcriptionally active at 500 mosmol/kg (associated with cell survival), but not at 700 mosmol/ka (associated with apoptosis). Reduction of p53 expression by p53 antisense oligonucleotide increases sensitivity of renal cells in culture to hyperosmotic stress caused by NaCl. The possible mechanisms of the protection action of p53 against hypertonic stress are discussed. (C) 2001 Published byElsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:59:26