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Titolo:
Increased concentrations of 14-3-3 epsilon, gamma and zeta isoforms in cerebrospinal fluid of AIDS patients with neuronal destruction
Autore:
Wakabayashi, H; Yano, M; Tachikawa, N; Oka, S; Maeda, M; Kido, H;
Indirizzi:
Univ Tokushima, Inst Enzyme Res, Div Enzyme Chem, Tokushima 7708503, JapanUniv Tokushima Tokushima Japan 7708503 me Chem, Tokushima 7708503, Japan Int Med Ctr Japan, AIDS Clin Ctr, Shinjuku Ku, Tokyo 1628655, Japan Int Med Ctr Japan Tokyo Japan 1628655 Shinjuku Ku, Tokyo 1628655, Japan Immunobiol Labs Co Ltd, Gunma 3750005, Japan Immunobiol Labs Co Ltd Gunma Japan 3750005 Co Ltd, Gunma 3750005, Japan
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 1-2, volume: 312, anno: 2001,
pagine: 97 - 105
SICI:
0009-8981(200110)312:1-2<97:ICO1EG>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-DEPENDENT ACTIVATOR; CREUTZFELDT-JAKOB-DISEASE; TRYPTOPHAN HYDROXYLASES; NEUROLOGICAL DISORDERS; MOLECULAR-CLONING; 14-3-3 PROTEIN; BRAIN; 14-3-3-PROTEINS; CELLS; PHOSPHORYLATION;
Keywords:
14-3-3; AIDS; cerebrospinal fluid; immunoassay; AIDS dementia complex; cytomegalovirus encephalitis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Kido, H Univ Tokushima, Inst Enzyme Res, Div Enzyme Chem, Kuramoto Cho 3-18-15, Tokushima 7708503, Japan Univ Tokushima Kuramoto Cho 3-18-15 Tokushima Japan 7708503 Japan
Citazione:
H. Wakabayashi et al., "Increased concentrations of 14-3-3 epsilon, gamma and zeta isoforms in cerebrospinal fluid of AIDS patients with neuronal destruction", CLIN CHIM A, 312(1-2), 2001, pp. 97-105

Abstract

Background: 14-3-3 proteins are major evolutionarily conserved cytosolic proteins that regulate signal transduction, apoptosis and neurotransmitter synthesis. Five homologous 14-3-3 isoforms, beta, gamma, zeta, epsilon and eta, are reported in mammalian neurones. To elucidate the diagnostic value of 14-3-3 in cerebrospinal fluid (CSF), a highly specific antibody against each isoform and studies on the isoform patterns in patients with neuronal destruction are needed, Methods: In this study, we raised isoform-specific antibodies against 14-3-3 proteins and established a semiquantitative methodof identification of each isoform by Western immunoblotting. Results: We found that three isoforms, 14-3-3 epsilon, gamma and zeta, appeared in the CSF of HIV patients with AIDS dementia complex or cytomegalovirus encephalitis, but not in AIDS patients without neurological symptoms or the non-HIV patients examined. The isoform patterns in AIDS patients were different fromthose reported in Creutzfeldt-Jakob disease and herpes simplex encephalitis, suggesting that the isoform patterns may facilitate the differential diagnosis. A high frequency of 14-3-3 in CSF was observed in seriously ill AIDS patients, particularly those with CD4 levels of less than 20 mm(3). Conclusion: These findings suggested that 14-3-3 proteins were released from destroyed neural cells and are useful real-time markers of the rate and amountof neural cell destruction in these patients. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:30:40