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Titolo:
Rapid high-performance liquid chromatography determination of lopinavir, anovel HIV-1 protease inhibitor, in human plasma
Autore:
Faux, J; Venisse, N; Olivier, JC; Bouquet, S;
Indirizzi:
CHU La Miletrie, Lab Pharmacocinet, F-86021 Poitiers, France CHU La Miletrie Poitiers France F-86021 ocinet, F-86021 Poitiers, France UFR Med & Pharm, Grp Rech Medicament & BHE, F-86034 Poitiers, France UFR Med & Pharm Poitiers France F-86034 & BHE, F-86034 Poitiers, France
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 7-8, volume: 54, anno: 2001,
pagine: 469 - 473
SICI:
0009-5893(200110)54:7-8<469:RHLCDO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; ULTRAVIOLET DETECTION; CEREBROSPINAL-FLUID; ANTIRETROVIRAL THERAPY; INFECTED PATIENTS; INDINAVIR; RITONAVIR; SAQUINAVIR; NELFINAVIR; QUANTIFICATION;
Keywords:
column liquid chromatography; therapeutic drug monitoring; HIV-1 protease inhibitor; lopinavir; ABT-378;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Bouquet, S CHU La Miletrie, Lab Pharmacocinet, BP 577, F-86021 Poitiers, France CHU La Miletrie BP 577 Poitiers France F-86021 oitiers, France
Citazione:
J. Faux et al., "Rapid high-performance liquid chromatography determination of lopinavir, anovel HIV-1 protease inhibitor, in human plasma", CHROMATOGR, 54(7-8), 2001, pp. 469-473

Abstract

Lopinavir is a new specific and potent HIV-1 protease inhibitor. A rapid high-performance liquid chromatographic method using UV detection, has been developed and validated for the analysis of lopinavir in plasma. This involved a single liquid-solid extraction on an OASIS (R) HLB column in the presence of an internal standard. Separation was achieved on a Xterra (R), C-8 (150 x 3.9 mm I. D.) column with a mobile phase consisting of acetonitrile and water (41:59, v/v). The detection wavelength was 210 nm. The assay was linear from 0.187 to 10.0 mug.mL(-1) and the limit of quantification was 0.187 mug.mL(-1). Mean recovery was ranged from 90.7% to 97.8% for lopinavir and 97.1% for the internal standard. Day to day precision and accuracy wereless than 9.6% and 7.3% respectively. This rapid and simple method can readily be used for drug monitoring of lopinavir, in HIV-1 infected patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:37:05