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Titolo:
Inhibition of Na+/Ca2+ exchange by KB-R7943, a novel selective antagonist,attenuates phosphoethanolamine and free fatty acid efflux in rat cerebral cortex during ischemia-reperfusion injury
Autore:
Pilitsis, JG; Diaz, FG; ORegan, MH; Phillis, JW;
Indirizzi:
Wayne State Univ, Sch Med, Dept Physiol, Detroit, MI 48201 USA Wayne StateUniv Detroit MI USA 48201 Dept Physiol, Detroit, MI 48201 USA Wayne State Univ, Sch Med, Dept Neurosurg, Detroit, MI 48201 USA Wayne State Univ Detroit MI USA 48201 pt Neurosurg, Detroit, MI 48201 USA Univ Detroit Mercy, Sch Dent, Detroit, MI 48219 USA Univ Detroit Mercy Detroit MI USA 48219 , Sch Dent, Detroit, MI 48219 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 916, anno: 2001,
pagine: 192 - 198
SICI:
0006-8993(20011019)916:1-2<192:IONEBK>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARACHIDONIC-ACID; PHOSPHOLIPASE A(2); INTRACELLULAR CALCIUM; NA+/H+ EXCHANGE; AMINO-ACIDS; RELEASE; BRAIN; CELLS; NEURONS; LIBERATION;
Keywords:
cerebral ischemia; free fatty acid; phospholipase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Phillis, JW Wayne State Univ, Sch Med, Dept Physiol, 540 E Canfield, Detroit, MI 48201USA Wayne State Univ 540 E Canfield Detroit MI USA 48201 48201USA
Citazione:
J.G. Pilitsis et al., "Inhibition of Na+/Ca2+ exchange by KB-R7943, a novel selective antagonist,attenuates phosphoethanolamine and free fatty acid efflux in rat cerebral cortex during ischemia-reperfusion injury", BRAIN RES, 916(1-2), 2001, pp. 192-198

Abstract

Reversal of the Na+/Ca+ exchanger (NCX) occurs during ischemia-reperfusioninjury as a result of changes in intracellular pH and sodium concentration. Inhibition of NCXs has been shown to be neuroprotective in vitro. In thisstudy, we evaluated the effects of KB-R7943 (50 muM), a specific inhibitorof the reverse mode of NCX, applied topically onto rat cerebral cortex prior to and during ischemia. Amino acid and free fatty acid levels in cortical superfusates, withdrawn at 10-min intervals from bilateral cortical windows, were analyzed by high-performance liquid chromatography. During a 20-min period of ischemia in control animals, there were significant increases in all amino acids and in all FFAs. Following reperfusion, all FFAs remainedsignificantly elevated, Application of KB-R7943 (50 muM) significantly inhibited effluxes of phosphoethanolamine, but had no effect on glutamate, aspartate, taurine or GABA levels. KB-R7943 also resulted in significant reductions in levels of myristic, docosahexaenoic and arachidonic acid during ischemia and in reperfusion levels of arachidonic and docosahexaenoic acids. These data indicate that inhibition of Na+/Ca2+ exchange likely prevented the activation of phospholipases that usually occurs following an ischemic insult as evidenced by its attenuation of phosphoethanolamine and free fattyacid efflux. The inhibition of phospholipases may be an essential component of the neuroprotective benefits of Na+/Ca2+ exchange inhibitors in ischemia-reperfusion injury and may provide a basis for their possible use in therapeutic strategies for stroke. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 29/01/20 alle ore 19:06:15