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Titolo:
Concentration-dependent effects of the esterase inhibitor BNPP on macrophage migration and myelin phagocytosis
Autore:
Siebert, H; Engelke, S; Maruschak, B; Bruck, W;
Indirizzi:
Klinikum Rudolf Virchow, Inst Neuropathol, Charite, D-13353 Berlin, Germany Klinikum Rudolf Virchow Berlin Germany D-13353 , D-13353 Berlin, Germany Univ Hosp Gottingen, Inst Neuropathol, D-37075 Gottingen, Germany Univ Hosp Gottingen Gottingen Germany D-37075 D-37075 Gottingen, Germany
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 916, anno: 2001,
pagine: 159 - 164
SICI:
0006-8993(20011019)916:1-2<159:CEOTEI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
WALLERIAN DEGENERATION; PERITONEAL-MACROPHAGES; SERINE ESTERASE-1; PERIPHERAL-NERVE; INVITRO; INJURY; RECRUITMENT; EXPRESSION; ALPHA;
Keywords:
Wallerian degeneration; carboxyl esterase; sciatic nerve; macrophage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Bruck, W Klinikum Rudolf Virchow, Inst Neuropathol, Charite, Augustenburger Pl 1, D-13353 Berlin, Germany Klinikum Rudolf Virchow Augustenburger Pl 1Berlin Germany D-13353
Citazione:
H. Siebert et al., "Concentration-dependent effects of the esterase inhibitor BNPP on macrophage migration and myelin phagocytosis", BRAIN RES, 916(1-2), 2001, pp. 159-164

Abstract

Wallerian degeneration of a peripheral nerve is mainly characterized by axon and myelin degradation and is paralleled by a massive invasion of peripheral macrophages into the nerve. These cells enter the nerve attracted by acascade of chemokines and cytokines but require proteolytic and enzymatic factors which enables them to cross the blood-nerve barrier. Here we investigated whether U-naphthyl (alpha -NA) esterases - which have been shown to be exclusively expressed in human monocytes - play a role during Wallerian degeneration. These enzymes were blocked by the specific inhibitor bis(4-nitrophenyl)-phosphate (BNPP) in an established in vitro model of Wallerian degeneration. Sciatic nerve segments of mice were co-cultured with peritoneal macrophages and BNPP was added to the cultures in various concentrations and at different timepoints. The macrophage numbers and myelin density in the nerve segments and the myelin load of macrophages were evaluated. After BNPP treatment the macrophage number within the nerve was significantly diminished and the myelin load within the macrophages was decreased, resultingin elevated levels of preserved myelin within the nerves. These experiments clearly showed a double effect of the UNA esterase inhibitor BNPP on macrophages, First, it suggests a role for UNA esterases on the migratory potential of macrophages since their invasion into the nerves was diminished. Second, the reduced myelin uptake is due to the inhibition of phagocytic capacity of these cells by BNPP. The therapeutical use of this inhibitor for treatment of autoimmune diseases such as multiple sclerosis or Guillain-Barresyndrome remains to be investigated. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 12/07/20 alle ore 09:08:36