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Titolo:
Identification of a novel integral plasma membrane protein induced during adipocyte differentiation
Autore:
Albrektsen, T; Richter, HE; Clausen, JT; Fleckner, J;
Indirizzi:
Novo Nordisk AS, Dept Transcript Biol, DK-2880 Bagsvaerd, Denmark Novo Nordisk AS Bagsvaerd Denmark DK-2880 ol, DK-2880 Bagsvaerd, Denmark Univ So Denmark, Dept Biochem & Mol Biol, DK-5230 Odense M, Denmark Univ So Denmark Odense Denmark M m & Mol Biol, DK-5230 Odense M, Denmark Novo Nordisk AS, Dept Signal Transduct, DK-2880 Bagsvaerd, Denmark Novo Nordisk AS Bagsvaerd Denmark DK-2880 ct, DK-2880 Bagsvaerd, Denmark Novo Nordisk AS, Dept Assay & Cell Technol, DK-2880 Bagsvaerd, Denmark Novo Nordisk AS Bagsvaerd Denmark DK-2880 ol, DK-2880 Bagsvaerd, Denmark
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 359, anno: 2001,
parte:, 2
pagine: 393 - 402
SICI:
0264-6021(20011015)359:<393:IOANIP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; MESSENGER-RNA LEVELS; 3T3-L1 PREADIPOCYTES; GENE-EXPRESSION; BINDING-PROTEIN; ADIPOSE DIFFERENTIATION; TARGET GENE; ADULT-RATS; OBESE GENE; CELL-LINE;
Keywords:
adipose tissue; differential display; 3T3-L1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Fleckner, J Novo Nordisk AS, Dept Transcript Biol, Novo Alle 6B2-83, DK-2880 Bagsvaerd, Denmark Novo Nordisk AS Novo Alle 6B2-83 Bagsvaerd Denmark DK-2880 rk
Citazione:
T. Albrektsen et al., "Identification of a novel integral plasma membrane protein induced during adipocyte differentiation", BIOCHEM J, 359, 2001, pp. 393-402

Abstract

Adipocyte differentiation is co-ordinately regulated by several transcription factors and is accompanied by changes in the expression of a variety ofgenes. Using mRNA differential display analysis, we have isolated a novel mRNA, DD16, specifically induced during the course of adipocyte differentiation. DD16 mRNAs are present in several tissues, but among the tissues tested, a remarkably higher level of expression was found in white adipose tissue. The DD16 cDNA encoded a polypeptide of 415 amino acids containing a single N-glycosylation site and an N-terminal hydrophobic stretch of 19 amino acids forming a transmembrane segment, indicating that DD16 is a glycosylated membrane-bound protein. Polyclonal antibodies raised against the DD16 peptide detected immunoreactive DD16 in membrane fractions, notably the plasma membrane, Association of DD16 with the plasma membrane was further confirmed by biotinylation studies of cell surface proteins, su suggesting that DD16 is an integral plasma membrane protein. Therefore we propose to give DD16 the name APMAP (Adipocyte Plasma Membrane Associated Protein). Although the biological function of this polypeptide is presently unknown, our data suggest that APMAP may function as a novel protein involved in the cross-talk of mature adipocytes with the environment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:46:49