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Titolo:
Overexpression of transcription factor Ets-1 in rheumatoid arthritis synovial membrane - Regulation of expression and activation by interleukin-1 andtumor necrosis factor alpha
Autore:
Redlich, K; Kiener, HP; Schett, G; Tohidast-Akrad, M; Selzer, E; Radda, I; Stummvoll, GH; Steiner, CW; Groger, M; Bitzan, P; Zenz, P; Smolen, JS; Steiner, G;
Indirizzi:
Univ Vienna, Vienna, Austria Univ Vienna Vienna AustriaUniv Vienna, Vienna, Austria Ludwig Boltzmann Inst Rheumatol, Vienna, Austria Ludwig Boltzmann Inst Rheumatol Vienna Austria eumatol, Vienna, Austria Hosp Baumgartner Hohe, Vienna, Austria Hosp Baumgartner Hohe Vienna Austria Baumgartner Hohe, Vienna, Austria
Titolo Testata:
ARTHRITIS AND RHEUMATISM
fascicolo: 2, volume: 44, anno: 2001,
pagine: 266 - 274
SICI:
0004-3591(200102)44:2<266:OOTFEI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE; MATRIX DEGRADATION; GENE-EXPRESSION; TNF-ALPHA; METALLOPROTEINASES; COLLAGENASE; FIBROBLASTS; MECHANISMS; CYTOKINES; FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Steiner, G Univ Hosp Vienna, Dept Internal Med 3, Div Rheumatol, WahringerGurtel 18-20, A-1090 Vienna, Austria Univ Hosp Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090
Citazione:
K. Redlich et al., "Overexpression of transcription factor Ets-1 in rheumatoid arthritis synovial membrane - Regulation of expression and activation by interleukin-1 andtumor necrosis factor alpha", ARTH RHEUM, 44(2), 2001, pp. 266-274

Abstract

Objective. To investigate the expression of the transcription factor Ets-1in synovial tissue and cultured synovial fibroblasts from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) and to study the regulationof Ets-1 expression and activation in synovial fibroblasts by proinflammatory cytokines. Methods. In situ expression of Ets-1 in synovial tissue from RA and OA patients was examined by double immunohistochemistry. The effects of interleukin-1 (IL-1) or tumor necrosis factor alpha (TNF alpha) on Ets-1 expression and activation (DNA binding) in cultured synovial fibroblasts were analyzedby Western blotting and DNA gel shift assay, respectively. In addition, the intracellular location of Ets-1 in synovial fibroblasts was determined byimmunofluorescence. Results. Pronounced expression of Ets-1 was detected in synovial tissues from all RA patients evaluated, particularly in the synovial lining layer and the sublining areas. Ets-1 was expressed by both fibroblasts and macrophages as well as by endothelial cells, while only a few T cells stained positive for Ets-1. In synovial specimens from OA patients, Ets-1 expression wasmuch less frequently observed and was largely restricted to vascular cells. Ets-1 was expressed to a similar degree in cultured synovial fibroblasts from RA and OA patients, as demonstrated by reverse transcriptase-polymerase chain reaction and Western blotting. Both IL-I and TNF alpha induced pronounced up-regulation of Ets-1 in synovial fibroblasts. Moreover, binding ofEts-1 to its specific DNA binding site was induced by both cytokines, although with different time courses. Immunofluorescence staining revealed a dominant nuclear localization of Ets-1 in IL-1- or TNF alpha -stimulated synovial fibroblasts. Conclusion. The overexpression of Ets-1 observed in RA synovial tissue appears to be caused by TNF alpha and IL-1, suggesting that Ets-1 may be an important factor in the cytokine-mediated inflammatory and destructive cascade characteristic of RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:58:40