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Titolo:
Fatty acid composition of freshwater heterotrophic flagellates: an experimental study
Autore:
Vera, A; Desvilettes, C; Bec, A; Bourdier, G;
Indirizzi:
Univ Clermont Ferrand, Lab Biol Protistes, UMR 6023, CNRS, F-63177 Clermont Ferrand, France Univ Clermont Ferrand Clermont Ferrand France F-63177 nt Ferrand, France
Titolo Testata:
AQUATIC MICROBIAL ECOLOGY
fascicolo: 3, volume: 25, anno: 2001,
pagine: 271 - 279
SICI:
0948-3055(20010928)25:3<271:FACOFH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACANTHAMOEBA-CASTELLANII; MARINE; MICROALGAE; ZOOPLANKTON; PROTOZOANS; BACTERIA; CULTURE; GROWTH; CARBON;
Keywords:
heterotrophic nanoflagellates; fatty acids; microbial loop; PUFA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Desvilettes, C Univ Clermont Ferrand, Lab Biol Protistes, UMR 6023, CNRS, F-63177 Clermont Ferrand, France Univ Clermont Ferrand Clermont Ferrand France F-63177 nce
Citazione:
A. Vera et al., "Fatty acid composition of freshwater heterotrophic flagellates: an experimental study", AQUAT MIC E, 25(3), 2001, pp. 271-279

Abstract

The fatty acid compositions of 5 freshwater heterotrophic flagellates werestudied using laboratory cultures. The flagellates Bodo saltans, Paraphysomonas vestita, Spumella pudica, Polytoma uvella and Chilomonas paramecium were cultured on Synura medium enriched either with Regilait milk powder (NIP medium; Regilait, St Martin Roche, France) or with Tabimin dry fish food (DFF medium; Tetra, Melle, France). The bacteria present in the culture media were also analyzed. The enrichment substrate was found to have a pronounced influence on the fatty acid profiles of the organisms. For example, flagellates grown on the MP medium were characterized by very low contents of polyunsaturated fatty acids (PUFA; 1.28 to 5.50% of total fatty acids), whereas those grown on DFF medium contained up to 13.20% PUFA. Irrespective ofthe species studied, the dominant PUFA were those of the (n-6) series. Thepresence of branched uneven-numbered fatty acids and of 18:1(n-7) within the protozoans reflects an intensive bacterivorous activity that is understandable in phagotrophic species such as B. saltans, P. vestita and S. pudicabut is debatable in C. paramecium and P. uvella, as these species have been described as osmotrophic. The heterotrophic flagellates in this study seemed to have varying, but rather limited, capacities of PUFA biosynthesis and bioconversion that were partly related to the physicochemical conditions in the cultures (temperature and dissolved oxygen) and to the phylogenetic relations of the organisms. Species containing a leucoplast or a residual plastid reproduced in a very attenuated manner the characteristic PUFA profiles of autotrophic flagellates belonging to their taxonomic group. This wasmainly the case for C. paramecium and P. uvella. Finally, our results suggest that in the natural environment, the PUFA contents of picoplanktonic prey and of dissolved organic matter are the main factors influencing the PUFA content of these flagellates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:41:48