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Titolo:
Production of native and recombinant lipases by Candida rugosa - A review
Autore:
Ferrer, P; Montesinos, JL; Valero, F; Sola, C;
Indirizzi:
Univ Autonoma Barcelona, Dept Engn Quim, ETSE, Bellaterra 08193, Barcelona, Spain Univ Autonoma Barcelona Bellaterra Barcelona Spain 08193 Barcelona, Spain
Titolo Testata:
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
fascicolo: 3, volume: 95, anno: 2001,
pagine: 221 - 255
SICI:
0273-2289(200109)95:3<221:PONARL>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-STATE FERMENTATION; FED-BATCH FERMENTATION; GEOTRICHUM-CANDIDUM; EXTRACELLULAR LIPASE; MICROBIAL LIPASES; BIOTECHNOLOGICAL APPLICATIONS; INTERFACIAL ACTIVATION; PSEUDOMONAS-AERUGINOSA; CHOLESTEROL ESTERASE; PARTIAL-PURIFICATION;
Keywords:
Candida rugosa; lipase; isoenzymes; fermentation; heterologous expression;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
152
Recensione:
Indirizzi per estratti:
Indirizzo: Ferrer, P Univ Autonoma Barcelona, Dept Engn Quim, ETSE, Bellaterra 08193,Barcelona, Spain Univ Autonoma Barcelona Bellaterra Barcelona Spain 08193 Spain
Citazione:
P. Ferrer et al., "Production of native and recombinant lipases by Candida rugosa - A review", APPL BIOC B, 95(3), 2001, pp. 221-255

Abstract

The yeast Candida rugosa produces multiple lipase isoenzymes sharing high sequence homology but with some differences in their catalytic properties. The regulation of C. rugosa lipase (CRL) synthesis and secretion in C. rugosa obeys a complex pattern. Fermentation processes for both wildtype and mutant C. rugosa strains are available for lipase production. Native CRL preparations have been extensively used for biotransformations. However, their inherent mixture of isoforms with variable profiles complicates interpretation and brings into question the reproducibility achieved between preparations. Although heterologous CRLs gene expression had been hampered owing to a nonuniversal codon usage, recent advances have made heterologous CRLs available. This will expand and improve the industrial utility of CRLs even further. The purpose of this review is to provide a summary of the recent advances on the production of native and recombinant lipases by C. rugosa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 04:45:09