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Titolo:
Effects of canine oviduct epithelial cells on movement and capacitation ofhomologous spermatozoa in vitro
Autore:
Kawakami, E; Kashiwagi, C; Hori, T; Tsutsui, T;
Indirizzi:
Nippon Vet & Anim Sci Univ, Dept Reprod, Musashino, Tokyo 1808602, Japan Nippon Vet & Anim Sci Univ Musashino Tokyo Japan 1808602 o 1808602, Japan
Titolo Testata:
ANIMAL REPRODUCTION SCIENCE
fascicolo: 1-2, volume: 68, anno: 2001,
pagine: 121 - 131
SICI:
0378-4320(20011031)68:1-2<121:EOCOEC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOG SPERM CAPACITATION; ACROSOME REACTION; ZONA-PELLUCIDA; IN-VITRO; BOVINE SPERMATOZOA; BOAR SPERM; REPRODUCTIVE-TRACT; EQUINE SPERMATOZOA; BULL SPERMATOZOA; CALCIUM INFLUX;
Keywords:
dog capacitation; hyperactivation; acrosome reaction; oviductal epithelial cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Kawakami, E Nippon Vet & Anim Sci Univ, Dept Reprod, 1-7-1 Kyonan Cho, Musashino, Tokyo 1808602, Japan Nippon Vet & Anim Sci Univ 1-7-1 Kyonan Cho Musashino Tokyo Japan 1808602
Citazione:
E. Kawakami et al., "Effects of canine oviduct epithelial cells on movement and capacitation ofhomologous spermatozoa in vitro", ANIM REPROD, 68(1-2), 2001, pp. 121-131

Abstract

In this study, the interaction between canine sperms and oviduct epithelial cells (OECs) was examined in vitro. The oviducts of eight bitches in the follicular (F-) phase and six bitches in the luteal (L-) phase were removedunder halothane inhalation anesthesia. The entire oviduct was opened longitudinally, and the oviductal epithelium of bitches in the F- and L-phases was scraped with a scalpel into tissue culture medium (Eagle's MEM) containing 10% estrous bitch serum and 10% diestrous bitch serum, respectively. TheOEC collected were preincubated for 24 h and then coincubated with ejaculated canine sperms at 38 degreesC under an atmosphere of 5% CO2 in air. The percentages of sperms exhibiting active tail movement (% TM), hyperactivated sperms (% HA), and acrosome-reacted sperms (% AR) were investigated until72 h after the start of coincubation. The percentage of sperms labeled with fluoresceinated Ca indicator (% Ca) was evaluated to assess the influx ofCa into sperms cytoplasm during capacitation. Canine sperms attached to both ciliated OEC and non-ciliated OEC. All of the mean % TM of the OEC-binding sperms in the F-OEC and L-OEC media after 24, 48, and 72 h of coincubation were significantly higher than the values of the freely swimming sperms (P less than or equal to 0.01). Conversely, the mean % AR and % Ca of the OEC-binding sperms were significantly lower (P < 0.01). All of the mean % H-A and % AR of the freely swimming sperms in the F-OEC medium after 24, 48, and 72 h of coincubation were significantly higher than the values of the sperms in the L-OEC medium (P < 0.01). These results indicate that attachment of canine sperms to the OEC prolongs their viability and motility and inhibits Ca influx into the sperms and sperm capacitation. These phenomena maybe responsible for maintaining the active movement and the fertile life ofcanine sperms in homologous oviducts. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:52:02