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Titolo:
CDNA cloning and physical mapping of porcine 3 beta-hydroxysteroid dehydrogenase/Delta 5-Delta 4 isomerase
Autore:
von Teichman, A; Joerg, H; Werner, P; Brenig, B; Stranzinger, G;
Indirizzi:
ETH Zentrum, Swiss Fed Inst Technol, Inst Anim Sci, Dept Anim Sci, CH-8092Zurich, Switzerland ETH Zentrum Zurich Switzerland CH-8092 m Sci, CH-8092Zurich, Switzerland St Lukes Roosevelt Hosp, New York, NY USA St Lukes Roosevelt Hosp New York NY USA Roosevelt Hosp, New York, NY USA Univ Gottingen, Inst Vet Med, D-3400 Gottingen, Germany Univ Gottingen Gottingen Germany D-3400 t Med, D-3400 Gottingen, Germany
Titolo Testata:
ANIMAL GENETICS
fascicolo: 5, volume: 32, anno: 2001,
pagine: 298 - 302
SICI:
0268-9146(200110)32:5<298:CCAPMO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEHYDROGENASE DELTA-5-DELTA-4 ISOMERASE; SOMATIC-CELL HYBRIDS; DEHYDROGENASE/DELTA(5)-DELTA(4) ISOMERASE; STEROID DEHYDROGENASE; 3-BETA-HSD GENE; EXPRESSION; LOCALIZATION; ASSIGNMENT; TISSUES; FAMILY;
Keywords:
3 gamma beta-hydroxysteroid dehydrogenase/isomerase; androstenone; boar taint; porcine chromosome 4; steroidogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Stranzinger, G ETH Zentrum, Swiss Fed Inst Technol, Inst Anim Sci, Dept Anim Sci, CH-8092Zurich, Switzerland ETH Zentrum Zurich Switzerland CH-8092urich, Switzerland
Citazione:
A. von Teichman et al., "CDNA cloning and physical mapping of porcine 3 beta-hydroxysteroid dehydrogenase/Delta 5-Delta 4 isomerase", ANIM GENET, 32(5), 2001, pp. 298-302

Abstract

The 3 beta -hydroxvsteroid dehydrogenase/Delta5-Delta4-isomerase (3 beta -HSD) enzymes are essential for the biosynthesis of steroid hormones. The 3 beta -HSD gene family has been reported to encode for different isoenzymes which function either as dehydrogenase/isomerase or as reductase. The 3 beta -HSD enzymes are involved in the formation of the pheromone androstenone (5 alpha -androst-16-ene-3-one) which contributes to the unpleasant odour present in the meat of uncastrated boars. An reverse-transcription-polymerase chain reaction (RT-PCR) probe from porcine testicular tissue of a 3 beta -HSD enzyme was used to screen a porcine adipose tissue cDNA library. Both strands of the positive clones were sequenced and the putative coding sequence of 1122 nucleotides encodes 374 amino acids. Comparison of the putativeopen reading frame with the bovine and the human type I homologues revealed 85.6 and 79.3% identity, respectively. Fluorescence in situ hybridization(FISH) was performed with a labelled PAC clone containing the gene of interest. The 3 beta -HSD gene was mapped to the porcine chromosome 4q16-4q21 which is in accordance with the comparative gene map.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 14:01:28