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Titolo:
Pre-conditioning activates adenosine utilization in a cost-effective way during myocardial ischaemia
Autore:
Wikstrom, G; Kavianipour, M; Ronquist, G; Waldenstrom, A;
Indirizzi:
Univ Uppsala Hosp, Dept Cardiol, Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden la Hosp, Dept Cardiol, Uppsala, Sweden Univ Umea Hosp, Dept Cardiol, S-90185 Umea, Sweden Univ Umea Hosp Umea Sweden S-90185 p, Dept Cardiol, S-90185 Umea, Sweden Univ Uppsala Hosp, Dept Clin Chem, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 n Chem, S-75185 Uppsala, Sweden
Titolo Testata:
ACTA PHYSIOLOGICA SCANDINAVICA
fascicolo: 2, volume: 173, anno: 2001,
pagine: 185 - 194
SICI:
0001-6772(200110)173:2<185:PAAUIA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENYLATE ENERGY-CHARGE; PROTEIN-KINASE-C; RABBIT HEART; INFARCT SIZE; 5'-NUCLEOTIDASE ACTIVITY; INTERSTITIAL ADENOSINE; GLOBAL-ISCHEMIA; RAT-HEART; MICRODIALYSIS; METABOLISM;
Keywords:
adenosine; glycolysis; hexose monophosphate pathway; inosine; lactate; microdialysis; pre-conditioning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Waldenstrom, A Dept Publ Hlth & Clin Med, S-90187 Umea, Sweden Dept Publ Hlth & Clin Med Umea Sweden S-90187 mea, Sweden
Citazione:
G. Wikstrom et al., "Pre-conditioning activates adenosine utilization in a cost-effective way during myocardial ischaemia", ACT PHYSL S, 173(2), 2001, pp. 185-194

Abstract

During pre-conditioning the interstitial concentration of adenosine, in contrast to lactate, presents a die-away curve-pattern for every successive episode of ischaemia. This die-away pattern might not necessarily be attributed to diminished adenosine production. The present study was undertaken toInvestigate whether pre-conditioning alters the metabolic turnover of adenosine as observed by the lactate production during ischaemia. Interstitial levels of metabolites In pre-conditioned (n = 21) and non-preconditioned (n= 21) porcine hearts were monitored with microdialysis probes Inserted in both Ischaemic and non-ischaemic tissue in an open chest heart model. Threesubgroups perturbated with either plain microdialysis buffer (control), buffer containing adenosine (375 muM), or buffer containing deoxyadenosine (375 muM) were studied. All animals were subjected to 90 min of equilibrium microdialysis before 40 min of regional myocardial Ischaemia and 120 min of reperfusion. Pre-conditioning consisted of four repetitive episodes of 10 min of ischaemia and 20 min of reperfusion. Significantly higher levels of Inosine and lactate were found in the ischaemic tissue of the pre-conditioned subgroup receiving adenosine (P < 0.05) compared with the other two subgroups receiving deoxyadenosine and plain buffer, respectively. This difference was only valid for pre-conditioned ischaemic myocardium, and hence equalamounts of inosine and lactate were produced in the non-preconditioned Ischaemic myocardium regardless of the presence of adenosine or deoxyadenosine. In the non-ischaemic myocardium baseline levels of metabolites were measured in all subgroups. Pre-conditioning favoured degradation of exogenous adenosine to Inosine successively ending up In enhanced lactate production. This was probably because of the involvement of the hexose monophosphate pathway in the pre-conditioned ischaemic myocardium. This route may therefore be supplementary In energy metabolism as a metabolic flow can be started byadenosine ending up in lactate without initial adenosine 5'-triphosphate (ATP) investment. Utilization of adenosine in this way may also explain the successive die-away pattern of adenosine seen in consecutive pre-conditioning cycles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:54:12