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Titolo:
Confocal laser scanning microscopic imaging of subcellular organelles, mRNA, protein products, and the microvessel environment
Autore:
Itoh, J; Matsuno, A; Yamamoto, Y; Kawai, K; Serizawa, A; Watanabe, K; Itoh, Y; Osamura, RY;
Indirizzi:
Tokai Univ, Labs Struct & Funct Res, Sch Med, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Teikyo Univ, Ichihara Hosp, Dept Neurosurg, Chiba 2990111, Japan Teikyo Univ Chiba Japan 2990111 sp, Dept Neurosurg, Chiba 2990111, Japan NKK Hosp, Div Pathol, Clin Lab, Kawasaki, Kanagawa 2100852, Japan NKK Hosp Kawasaki Kanagawa Japan 2100852 awasaki, Kanagawa 2100852, Japan Tokai Univ, Sch Med, Div Diagnost Pathol, Isehara, Kanagawa 2591193, JapanTokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Aobagakuen Jr Coll, Setagaya Ku, Tokyo 1540017, Japan Aobagakuen Jr Coll Tokyo Japan 1540017 Setagaya Ku, Tokyo 1540017, Japan Tokai Univ, Dept Pathol, Sch Med, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan
Titolo Testata:
ACTA HISTOCHEMICA ET CYTOCHEMICA
fascicolo: 4, volume: 34, anno: 2001,
pagine: 285 - 297
SICI:
0044-5991(2001)34:4<285:CLSMIO>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; BIOTINYLATED OLIGONUCLEOTIDE PROBES; ANTIHEMOPHILIC FACTOR ANTIGEN; HUMAN-ENDOTHELIAL CELLS; HORMONE MESSENGER-RNA; RAT PITUITARY-GLANDS; 3-DIMENSIONAL RECONSTRUCTION; ELECTRON-MICROSCOPE; ULTRASTRUCTURAL DISTRIBUTION; INSITU HYBRIDIZATION;
Keywords:
confocal laser scanning microscopy; immunohistochemistry; in situ hybridization; microvessel environment; hormone-secreting cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Itoh, J Tokai Univ, Labs Struct & Funct Res, Sch Med, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 Kanagawa 2591193, Japan
Citazione:
J. Itoh et al., "Confocal laser scanning microscopic imaging of subcellular organelles, mRNA, protein products, and the microvessel environment", ACT HIST CY, 34(4), 2001, pp. 285-297

Abstract

The confocal laser scanning microscope (CLSM) was employed to visualize highly contrasted images of histochemically stained subcellular organelles. In early studies, only fluorescent signals were detectable by CLSM, however,recent innovations have enabled the visualization of non-fluorescent probes such as horseradish peroxidase (HRP)-diamino-benzidine (DAB). The non-fluorescent signals are permanently preserved and allow repeated or retrospective examinations. We applied CLSM to specimens prepared for light microscopy, and visualized subcellular organelles and pituitary hormone mRNA. The images were comparable to those obtained by electron microscopy (EM). We alsoapplied this technique to the observation of tumor angiogenesis and the microvessel environment of hormone-secreting cells. Both the visualization ofsubcellular organelles, mRNA and protein products, and 3D images of the microvessel environment of hormone-secreting cells are discussed in this review.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:24:54