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Titolo:
Systematic analysis of supersaturation and lysozyme crystal quality
Autore:
Yoshizaki, I; Sato, T; Igarashi, N; Natsuisaka, M; Tanaka, N; Komatsu, H; Yoda, S;
Indirizzi:
Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Innovat & Engineered Mat, Midori Ku, Yokohama, Kanagawa 2268502, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268502 anagawa 2268502, Japan Natl Space Dev Agcy Japan, Space Utilizat Res Ctr, Tsukuba, Ibaraki 3058505, Japan Natl Space Dev Agcy Japan Tsukuba Ibaraki Japan 3058505 ki 3058505, Japan Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 anagawa 2268501, Japan High Energy Accelerator Res Org, Inst Mat Struct Sci, Tsukuba, Ibaraki 3050801, Japan High Energy Accelerator Res Org Tsukuba Ibaraki Japan 3050801 0801, Japan Iwate Prefectural Univ, Takizawa, Iwate 0200193, Japan Iwate Prefectural Univ Takizawa Iwate Japan 0200193 Iwate 0200193, Japan
Titolo Testata:
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
, volume: 57, anno: 2001,
parte:, 11
pagine: 1621 - 1629
SICI:
0907-4449(200111)57:<1621:SAOSAL>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATOMIC-FORCE MICROSCOPY; EGG-WHITE LYSOZYME; TETRAGONAL LYSOZYME; GROWTH; TEMPERATURE; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Yoshizaki, I Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Innovat & Engineered Mat, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268502, Japan Tokyo Inst Technol 4259 Nagatsuta Yokohama Kanagawa Japan 2268502
Citazione:
I. Yoshizaki et al., "Systematic analysis of supersaturation and lysozyme crystal quality", ACT CRYST D, 57, 2001, pp. 1621-1629

Abstract

A systematic study of the correlation between supersaturation and protein crystal quality was carried out employing atomic force microscopy (AFM) andX-ray crystallography with synchrotron radiation (SR). The surface morphology and growth rates of hen egg-white (HEW) lysozyme crystals soaked in various supersaturated solutions were first investigated by AFM. The results showed that the formation of two-dimensional islands increased as a functionof supersaturation. The growth rate (molecule intake speed) also increasedas a function of supersaturation. In order to examine the correlation between the surface morphology, growth rate and the crystal quality, X-ray diffraction experiments were performed. It was confirmed that crystals grown atlower supersaturations diffracted better with higher signal-to-noise ratios, including better agreement between symmetry-related reflections. The results strongly suggested that the molecular misorientation at high supersaturation affected the crystal quality.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:43:29