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Titolo:
Cloning of a fatty acid synthase component FAS1 gene from Saccharomyces kluyveri and its functional complementation of S-cerevisiae fas1 mutant
Autore:
Kajiwara, S; Oura, T; Shishido, K;
Indirizzi:
Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 anagawa 2268501, Japan
Titolo Testata:
YEAST
fascicolo: 14, volume: 18, anno: 2001,
pagine: 1339 - 1345
SICI:
0749-503X(200110)18:14<1339:COAFAS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; YEAST; SUBUNIT; SYNTHETASE; PROTEIN; LENGTH; ENZYME;
Keywords:
fatty acid synthesis; Saccharomyces kluyveri; fas1 complementation; heterologous expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Kajiwara, S Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Life Sci, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol 4259 Nagatsuta Yokohama Kanagawa Japan 2268501
Citazione:
S. Kajiwara et al., "Cloning of a fatty acid synthase component FAS1 gene from Saccharomyces kluyveri and its functional complementation of S-cerevisiae fas1 mutant", YEAST, 18(14), 2001, pp. 1339-1345

Abstract

A gene encoding a fatty acid synthase component, FAS1, has been cloned from a genomic library of the polyunsaturated fatty acid (PUFA)-producing yeast Saccharomyces kluyveri. This gene (named Sk-FAS1) was found to contain anopen reading frame of 6150 bp, coding for 2049 amino acids. The deduced Sk-FAS1 protein showed significant (75-59%) homology with FAS proteins from the other yeasts, including S. cerevisiae, Candida albicans and Yarrowia lipolytica. The substrate-binding sites of the acetyl transferase and malonyl/palmitoyl transferase domains, and the FMN- and NADPH-binding sites of the enoyl reductase domain, were all highly conserved. Expression of the Sk-FAS1 gene in S. cerevisiae complemented genetic disruption of the S. cerevisiae FAS1 gene (Sc-FAS1), suggesting the formation of a heterogeneous complex of Sk-FAS1 (beta) and Sc-FAS2 (alpha), which is able to function to synthesize fatty acids. Compared with the isogenic wild-type of S. cerevisiae, as well as S. kluyveri, the S. cerevisiae fas1 mutant carrying the Sk-FAS1 gene showed an increase in the relative amount of 16-carbon fatty acids and a decrease in 18-carbon fatty acids. The DDBJ Accession No. for the sequence reported in this paper is AB054690. Copyright (C) 2001 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/11/20 alle ore 03:16:34