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Titolo:
Gender differences in cytokine secretion by human peripheral blood mononuclear cells: Role of estrogen in modulating LPS-induced cytokine secretion in an ex vivo septic model
Autore:
Asai, K; Hiki, N; Mimura, Y; Ogawa, T; Unou, K; Kaminishi, M;
Indirizzi:
Univ Tokyo, Dept Surg, Bunkyo Ku, Tokyo 1128688, Japan Univ Tokyo Tokyo Japan 1128688 ept Surg, Bunkyo Ku, Tokyo 1128688, Japan
Titolo Testata:
SHOCK
fascicolo: 5, volume: 16, anno: 2001,
pagine: 340 - 343
SICI:
1073-2322(200111)16:5<340:GDICSB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; SEPSIS SYNDROME; BINDING-SITES; SHOCK; LIPOPOLYSACCHARIDE; MORTALITY; RECEPTORS; MONOCYTES; SERUM; MACROPHAGES;
Keywords:
sex differences; estradiol; endotoxin; cytokine; healthy volunteer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Hiki, N Univ Tokyo, Dept Surg, Bunkyo Ku, 3-28-6 Mejirodai, Tokyo 1128688,Japan Univ Tokyo 3-28-6 Mejirodai Tokyo Japan 1128688 yo 1128688, Japan
Citazione:
K. Asai et al., "Gender differences in cytokine secretion by human peripheral blood mononuclear cells: Role of estrogen in modulating LPS-induced cytokine secretion in an ex vivo septic model", SHOCK, 16(5), 2001, pp. 340-343

Abstract

Clinical studies demonstrate a better outcome of sepsis in females. Elevated estrogen levels and plasma cytokine imbalance occur in septic patients. We propose that gender-different cytokine secretion by the peripheral bloodmononuclear cells (PBMCs) in sepsis determines the clinical outcome. A 2 x10(6) PBMC sample from healthy volunteers (10 males and 10 females) was incubated with 1 ng/mL of lipopolysaccharide (LPS), estradiol (E-2; 0, 0.03, 0.3, 3.0, 30 ng/mL), or 1 ng/mL of LPS + E-2 (0, 0.03, 0.3, 3.0, 30 ng/ml),and supernatant cytokine levels were measured. Tumor necrosis factor alpha(TNF alpha) and interleukin (IL)-6 production by PBMCs from both sexes wastime-dependently stimulated by LPS. At 6 h after LPS challenge, the TNF alpha level of male PBMCs was significantly higher but IL-6 secretion by female PBMCs was higher (two-way ANOVA: P<0.05). E-2 alone stimulated cytokine secretion by male PBMCs. Addition of the same E-2 concentration as in sepsis patients' plasma modulated LPS-induced cytokine production. No significant sex differences in LPS-stimulated TNF<alpha> or IL-6 secretion by PBMCs were found, but IL-10 secretion by male PBMCs was significantly suppressed. This study demonstrated a gender difference in PBMCs responsiveness to LPS and E-2 stimulation and E-2-modulated cytokine secretion. In this PBMCs model of sepsis, only the supernatant IL-10 level was significantly lower in males. These ex vivo findings may partially explain the mechanism underlyingthe poorer outcome of male sepsis patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/02/20 alle ore 17:24:04