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Titolo:
Poly(A)-binding proteins regulate both mRNA deadenylation and decapping inyeast cytoplasmic extracts
Autore:
Wilusz, CJ; Gao, M; Jones, CL; Wilusz, J; Peltz, SW;
Indirizzi:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey Piscataway NJ USA 08854 scataway, NJ 08854 USA Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Microbiol & Mol Genet, Newark, NJ 07103 USA Univ Med & Dent New Jersey Newark NJ USA 07103 enet, Newark, NJ 07103 USA
Titolo Testata:
RNA-A PUBLICATION OF THE RNA SOCIETY
fascicolo: 10, volume: 7, anno: 2001,
pagine: 1416 - 1424
SICI:
1355-8382(200110)7:10<1416:PPRBMD>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA TURNOVER; TRANSLATION INITIATION-FACTORS; SACCHAROMYCES-CEREVISIAE; IN-VITRO; DEPENDENT TRANSLATION; SPLICING INVITRO; BINDING-PROTEIN; RATES; DECAY; PAB1P;
Keywords:
eIF4E; mRNA decay; poly(A) tail; translation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Peltz, SW Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, 675 Hoes Lane, Piscataway, NJ 08854 USA Univ Med & Dent New Jersey 675 Hoes Lane Piscataway NJ USA 08854
Citazione:
C.J. Wilusz et al., "Poly(A)-binding proteins regulate both mRNA deadenylation and decapping inyeast cytoplasmic extracts", RNA, 7(10), 2001, pp. 1416-1424

Abstract

The pathway of mRNA degradation has been extensively studied in the yeast,Saccharomyces, cerevisiae, and it is now clear that many mRNAs decay by a deadenylation-dependent mechanism. Although several of the factors requiredfor mRNA decay have been identified, the regulation and: precise roles of many of the proteins involved remains unclear. We have developed an in vitro system that recapitulates both the deadenylation and the decapping steps of mRNA decay. Furthermore, both deadenylation and decapping are inhibited by poly(A) binding proteins in our assay. Our system has allowed us to separate the decay process from translation and we have shown that the poly(A) tail is capable of inhibiting decapping in an elF4E-independent manner. Ourin vitro system should prove invaluable in dissecting the mechanisms of mRNA turnover.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 02:08:44