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Titolo:
Transport and distribution of benzyladenine in Actinidia deliciosa explants cultured in liquid and solid media
Autore:
Feito, I; Gonzalez, A; Centeno, ML; Fernandez, B; Rodriguez, A;
Indirizzi:
Univ Oviedo, Lab Fisiol Vegetal, Dept Biol Organismos & Sistemas, Fac Biol, E-33071 Oviedo, Spain Univ Oviedo Oviedo Spain E-33071 stemas, Fac Biol, E-33071 Oviedo, Spain Dept Hortofruticultura, Serv Reg Invest & Desarrollo Agroalimentari, Villaviciosa 33300, Asturias, Spain Dept Hortofruticultura Villaviciosa Asturias Spain 33300 Asturias, Spain Univ Santiago de Compostela, Dept Biol Vegetal, Escuela Politecn Super, Lugo 27002, Spain Univ Santiago de Compostela Lugo Spain 27002 cn Super, Lugo 27002, Spain
Titolo Testata:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 10, volume: 39, anno: 2001,
pagine: 909 - 916
SICI:
0981-9428(200110)39:10<909:TADOBI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOKININ CONCENTRATION; ENDOGENOUS CYTOKININS; MINERAL-NUTRITION; APICAL DOMINANCE; ZEATIN RIBOSIDE; SSP PLEIOSPERMA; TISSUE-CULTURE; ABSCISIC-ACID; PEA-PLANTS; CELL-CYCLE;
Keywords:
apoplast; autoradiography; cytokinin transport; kiwifruit; N-6-benzyladenine; symplast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Rodriguez, A Univ Oviedo, Lab Fisiol Vegetal, Dept Biol Organismos & Sistemas, Fac Biol, C Catedrat Rodrigo Uria S-N, E-33071 Oviedo, Spain Univ Oviedo C Catedrat Rodrigo Uria S-N Oviedo Spain E-33071
Citazione:
I. Feito et al., "Transport and distribution of benzyladenine in Actinidia deliciosa explants cultured in liquid and solid media", PL PHYS BIO, 39(10), 2001, pp. 909-916

Abstract

The effect of the culture medium on the transport and distribution of N-6-benzyladenine along the microshoots of kiwifruit cultured on solid and liquid media in the presence of 8-[C-14]-BA was studied. Three complementary approaches were used: quantification of the radioactivity present in the different portions in which the explants were divided; distribution of BA and its metabolites in apoplast and symplast; and autoradiography. In both media, there was a gradual decrease of BA content from the basal to the apical segment, but this decrease was more uniform in explants from liquid media than from solid media, where a higher accumulation in the first two (more basal) segments was observed. Apoplast/symplast distribution of BA showed thatBA and its metabolites were localized both in the symplast and in the apoplast. However, the major radioactive fraction was associated with the symplast, where the explants cultured in liquid medium had most of the BA activeforms (BA and BAR). In the explants cultured in solid medium, the proportion of active and inactive (BA9G, BA3G and BA7G) forms was similar. The autoradiography of buds development showed a major concentration of radioactivity in those grown in liquid medium. Radioactivity distribution showed a similar pattern in the explants from both media, nevertheless the density of labelling was higher in meristematic than in differentiated zones. In conclusion, the explants from liquid medium have a higher BA content, its inactivation is slower, its distribution is more effective, and its presence in the meristematic tissue is also greater than in the solid medium. Therefore, it seems that BA might be responsible for different developmental patterns observed in explants grown in both media types. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 11:37:58