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Titolo:
The first exon coding region of cystathionine gamma-synthase gene is necessary and sufficient for downregulation of its own mRNA accumulation in transgenic Arabidopsis thaliana
Autore:
Suzuki, A; Shirata, Y; Ishida, H; Chiba, Y; Onouchi, H; Naito, S;
Indirizzi:
Hokkaido Univ, Grad Sch Agr, Div Appl Biosci, Sapporo, Hokkaido 0608589, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608589 oro, Hokkaido 0608589, Japan
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 10, volume: 42, anno: 2001,
pagine: 1174 - 1180
SICI:
0032-0781(200110)42:10<1174:TFECRO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA STABILITY; AGROBACTERIUM-MEDIATED TRANSFORMATION; GREEN FLUORESCENT PROTEIN; METHIONINE BIOSYNTHESIS; SOLUBLE METHIONINE; HIGHER-PLANTS; BETA-TUBULIN; GUS FUSIONS; TRANSLATION; DEGRADATION;
Keywords:
Arabidopsis thaliana; cystathionine gamma-synthase (EC 4.2.99.9); feedback regulation; methionine biosynthesis; mRNA stability; transgenic expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Naito, S Hokkaido Univ, Grad Sch Agr, Div Appl Biosci, Sapporo, Hokkaido 0608589, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608589 aido 0608589, Japan
Citazione:
A. Suzuki et al., "The first exon coding region of cystathionine gamma-synthase gene is necessary and sufficient for downregulation of its own mRNA accumulation in transgenic Arabidopsis thaliana", PLANT CEL P, 42(10), 2001, pp. 1174-1180

Abstract

Expression of the gene for cystathionine gamma -synthase (CGS), which catalyzes the key step of methionine biosynthesis, is feedback regulated at thelevel of mRNA stability. The first exon polypeptide of CGS is suggested tobe involved in this regulation and amino acid sequence alterations caused by mto1 mutations in that region lead to an overaccumulation of CGS mRNA [Chiba et al. (1999) Science 286: 1371-1374]. Transgenic Arabidopsis thalianaharboring chimeric constructs in which wild-type or mto1 mutant CGS exon 1are fused in-frame to reporter genes and driven by the cauliflower mosaic virus 35S RNA promoter were constructed. Studies with these transgenic lines demonstrated that the coding region of CGS exon 1 is necessary and sufficient for downregulation of its own mRNA accumulation in response to methionine application and that this region acts in cis in this process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:40:56