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Titolo:
Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants
Autore:
Furuichi, T; Mori, IC; Takahashi, K; Muto, S;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, JapanNagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ, Biosci Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ, Sch Informat & Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 10, volume: 42, anno: 2001,
pagine: 1149 - 1155
SICI:
0032-0781(200110)42:10<1149:SIICCI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC TOBACCO PLANTS; CALCIUM CHANNELS; PLASMA-MEMBRANE; EXPRESSION; SUCROSE; PROTEIN; GENE; POTATO; CELLS; TRANSFORMATION;
Keywords:
aequorin; Arabidopsis thaliana; calcium signaling; luminescence imaging; sucrose-H+ symporter; sugar signal transduction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Muto, S Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 goya, Aichi 4648601, Japan
Citazione:
T. Furuichi et al., "Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants", PLANT CEL P, 42(10), 2001, pp. 1149-1155

Abstract

Using Ca2+-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca2+ concentration ([Ca2+](cyt)) was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose roots hadbeen incubated overnight with coelenterazine to reconstitute aequorin systemically, strong and transient (within 20 s) luminescence was observed in the roots; that luminescence was followed by weak luminescence moving from the lower leaves to the upper leaves. The moving rate of luminescence was roughly comparable to that of [C-14]sucrose. Application of 0.1 M glucose or fructose induced transient luminescence in excised leaves. No such luminescence was observed in heterotrophically grown (with sucrose) whole plants orin excised tissues. mRNA levels of sucrose-H+ symporter genes AtSUC1 and AtSUC2 were higher in autotrophic plants than in heterotrophic plants. Theseresults indicate that influx of transported sucrose together with H+ into the mesophyll cells of autotrophic plants may depolarize the membrane potential, and subsequently activate a voltage-gated Ca2+ channel on the plasma membrane, resulting in a [Ca2+](cyt) increase. The [Ca2+](cyt) increase might initiate Ca2+ signaling leading to the expression of genes related to biosynthesis of storage carbohydrates. Hexoses, when applied, might also be involved in the [Ca2+](cyt) increase mediated by monosaccharide-H+ co-transporters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:03:12