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Titolo:
Early postnatal dexamethasone diminishes transforming growth factor alpha localization within the ileal muscularis propria of newborn mice and extremely low-birth-weight infants
Autore:
Gordon, PV; Price, WA; Stiles, AD; Rutledge, JC;
Indirizzi:
Univ Virginia Hlth Syst, Dept Pediat, Div Neonatol, Charlottesville, VA 22908 USA Univ Virginia Hlth Syst Charlottesville VA USA 22908 sville, VA 22908 USA Univ N Carolina, Dept Pediat, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 ediat, Chapel Hill, NC 27599 USA Childrens Hosp & Reg Med Ctr, Dept Labs, Seattle, WA 98115 USA Childrens Hosp & Reg Med Ctr Seattle WA USA 98115 , Seattle, WA 98115 USA
Titolo Testata:
PEDIATRIC AND DEVELOPMENTAL PATHOLOGY
fascicolo: 6, volume: 4, anno: 2001,
pagine: 532 - 537
SICI:
1093-5266(200111/12)4:6<532:EPDDTG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYPERTROPHIC PYLORIC-STENOSIS; MILK; RAT; EXPRESSION; DISEASE;
Keywords:
transforming growth factor alpha; epidermal growth factor; ileum; development; smooth muscle; spontaneous intestinal perforation; focal small bowel perforation; extremely low-birth-weight infant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Gordon, PV Univ Virginia Hlth Syst, Dept Pediat, Div Neonatol, POB 800386,Charlottesville, VA 22908 USA Univ Virginia Hlth Syst POB 800386 Charlottesville VA USA 22908
Citazione:
P.V. Gordon et al., "Early postnatal dexamethasone diminishes transforming growth factor alpha localization within the ileal muscularis propria of newborn mice and extremely low-birth-weight infants", PEDIATR D P, 4(6), 2001, pp. 532-537

Abstract

Focal small bowel perforation (FSBP) occurs most commonly in the ileum of extremely low-birth-weight (ELBW) infants. Early postnatal dexamethasone (EPD) administration results in an increased risk for FSBP in this patient population, but the mechanism by which this occurs is unknown. Infants with FSBP have healthy mucosa but thinned smooth muscle, suggesting a mechanism involving the muscularis propria for these perforations. One explanation forthese findings would be that dexamethasone alters the tissue availability of pertinent growth factors to the smooth muscle. To explore this possibility, we administered dexamethasone or saline by intraperitoneal injection tonewborn mice for 3 days (dosed at 1 mug/g of body weight/day) to simulate EPD protocols. The animals were sacrificed after 72 h of treatment and their ileums harvested and prepared for microscopy. Immunolocalization was performed for three related growth factors (epidermal growth factor [EGF], heparin-binding EGF [h-EGF], and transforming growth factor a [TGF-alpha]) and their common receptor. We found TGF-alpha to be abundant and discretely localized in the muscularis propria in control animals but to be diminished indexamethasone-treated animals. EGF-receptor immunostaining was also decreased with dexamethasone but there was minimal to no detection of EGF or h-EGF in either treatment condition. Surgical and autopsy specimens of the ileum were obtained from seven ELBW infants who either received EPD or not. These tissues were used for immunolocalization of the same growth factors and similar distributions for TGF-alpha were observed in several of these cases. These findings are consistent with an autocrine role for TGF-alpha in ileal smooth muscle proliferation and suggest a mechanism by which EPD might mediate smooth muscle thinning.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:13:32