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Titolo:
A novel function of tissue factor pathway inhibitor-2 (TFPI-2) in human glioma invasion
Autore:
Konduri, SD; Rao, CN; Chandrasekar, N; Tasiou, A; Mohanam, S; Kin, Y; Lakka, SS; Dinh, D; Olivero, WC; Gujrati, M; Foster, DC; Kisiel, W; Rao, JS;
Indirizzi:
Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 ci, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Neurosurg, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 rg, Div Canc Biol, Peoria, IL 61656 USA Univ Illinois, Coll Med, Dept Neuropathol, Div Canc Biol, Peoria, IL 61656USA Univ Illinois Peoria IL USA 61656 hol, Div Canc Biol, Peoria, IL 61656USA Zymogenet Inc, Seattle, WA 98105 USA Zymogenet Inc Seattle WA USA 98105Zymogenet Inc, Seattle, WA 98105 USA Univ New Mexico, Hlth Sci Ctr, Albuquerque, NM 87131 USA Univ New Mexico Albuquerque NM USA 87131 i Ctr, Albuquerque, NM 87131 USA Ctr Prostate Dis Res, Rockville, MD USA Ctr Prostate Dis Res Rockville MDUSA rostate Dis Res, Rockville, MD USA
Titolo Testata:
ONCOGENE
fascicolo: 47, volume: 20, anno: 2001,
pagine: 6938 - 6945
SICI:
0950-9232(20011018)20:47<6938:ANFOTF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX-DEGRADING METALLOPROTEINASE; SERINE PROTEINASE-INHIBITORS; ENDOTHELIAL GROWTH-FACTOR; PLASMINOGEN-ACTIVATOR; CELL-LINE; PROTEASE INHIBITOR; MELANOMA-CELLS; CDNA CLONING; FACTOR VIIA; IN-SITU;
Keywords:
TFPI-2; glioblastoma; invasion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS Univ Illinois, Coll Med, Dept Biomed & Therapeut Sci, Div Canc Biol, 1 Illini Dr, Peoria, IL 61656 USA Univ Illinois 1 Illini Dr Peoria IL USA 61656 Peoria, IL 61656 USA
Citazione:
S.D. Konduri et al., "A novel function of tissue factor pathway inhibitor-2 (TFPI-2) in human glioma invasion", ONCOGENE, 20(47), 2001, pp. 6938-6945

Abstract

Human tissue factor pathway inhibitor-2 (TFPI-2) is a Kunitz-type serine protease inhibitor that inhibits plasmin, trypsin, chymotrypsin, cathepsin G, and plasma kallikrein but not urokinase-type plasminogen activator, tissue plasminogen activator, or thrombin. Preliminary findings in our laboratory suggested that the expression of TFPI-2 is downregulated or lost during tumor progression in human gliomas. To investigate the role of TFPI-2 in theinvasiveness of brain tumors, we stably transfected the human high-grade glioma cell line SNB19 and the human low-grade glioma cell line Hs683 with avector capable of expressing a transcript complementary to the full-lengthTFPI-2 mRNA in either sense (0.7 kb) or antisense (1 kb) orientations. Parental cells and stably transfected cell lines were analysed for TFPI-2 protein by Western blotting and for TFPI-2 mRNA by Northern blotting. The levels of TFPI-2 protein and mRNA were higher in the sense clones (SNB19) and decreased in the antisense (Hs683) clones than in the corresponding parental and vector controls. In spheroid and matrigel invasion assays, the SNB19 parental cells were highly invasive, but the sense-transfected SNB-19 clones were much less invasive; the antisense-transfected Hs683 clones were more invasive than their parental and vector controls. After intracerebral injection in mice, the sense-transfected SNB19 clones were less able to form tumors than were their parental and vector controls, and the antisense-Hs683 clones but not the parental or vector controls formed small tumors. This is the first study to demonstrate that down- or upregulation of TFPI-2 plays a significant role in the invasive behavior of human gliomas.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 21:36:39