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Titolo:
Metabotropic glutamate receptor 5 mediates the potentiation of N-methyl-D-aspartate responses in medium spiny striatal neurons
Autore:
Pisani, A; Gubellini, P; Bonsi, P; Conquet, F; Picconi, B; Centonze, D; Bernardi, G; Calabresi, P;
Indirizzi:
Univ Roma Tor Vergata, Dipartimento Neurosci, Neurol Clin, I-00133 Rome, Italy Univ Roma Tor Vergata Rome Italy I-00133 eurol Clin, I-00133 Rome, Italy Fdn Santa Lucia, IRCCS, Rome, Italy Fdn Santa Lucia Rome ItalyFdn Santa Lucia, IRCCS, Rome, Italy CNR, Ist Neurobiol & Med Mol, Rome, Italy CNR Rome ItalyCNR, Ist Neurobiol & Med Mol, Rome, Italy Glaxo Wellcome Expt Res, Inst Biol Cellulaire & Morphol, Lausanne, Switzerland Glaxo Wellcome Expt Res Lausanne Switzerland hol, Lausanne, Switzerland
Titolo Testata:
NEUROSCIENCE
fascicolo: 3, volume: 106, anno: 2001,
pagine: 579 - 587
SICI:
0306-4522(2001)106:3<579:MGR5MT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; RAT HIPPOCAMPAL SLICES; SUBUNIT MESSENGER-RNAS; MGLUR1 MUTANT MICE; NMDA-RESPONSES; HUNTINGTONS-DISEASE; SYNAPTIC PLASTICITY; (S)-4C3HPG PROTECTS; QUINOLINIC ACID; ACTIVATION;
Keywords:
electrophysiology; striatum; metabotropic glutamate receptor; basal ganglia; knockout mice; excitotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Pisani, A Univ Roma Tor Vergata, Dipartimento Neurosci, Neurol Clin, Via Tor Vergata135, I-00133 Rome, Italy Univ Roma Tor Vergata Via Tor Vergata 135 Rome Italy I-00133 ly
Citazione:
A. Pisani et al., "Metabotropic glutamate receptor 5 mediates the potentiation of N-methyl-D-aspartate responses in medium spiny striatal neurons", NEUROSCIENC, 106(3), 2001, pp. 579-587

Abstract

Medium spiny neurons were recorded from striatal slices obtained from micelacking the group I metabotropic glutamate receptor (mGluR) subtype 1 or subtype 5. In wild-type animals, N-methyl-D-aspartate (NMDA)-induced membrane depolarization/inward currents were potentiated in the presence of both the group I mGluR agonist 3,5-dihydroxyphenylglycine (3,5-DHPG) and the mGluR5 selective agonist (RS)-2-chloro-5-hydroxyphenylglycine (CHPG). Likewise.in mGluR1 knockout mice, both 3.5-DHPG and CHPG were able to potentiate NMDA responses. Conversely. in neurons recorded from mCluR5-deficient mice, the enhancement of NMDA responses by both 3,5-DHPG and CHPG was absent. Pharmacological analysis performed from rat slices confirmed the data obtained with mice. In the presence of the competitive mGluR1 antagonist LY367385, the NMDA responses were potentiated in the presence of CHPG, whereas the CHPG-induced enhancement was not observed in slices treated with the non-competitive mGluR5 antagonist 2-methyl-6-(phenylethynyl)-pyridine. As in wild-type mice, in neither of the mGluR1- and mGluR5-deficient mice did (2S,1' R.2' R,3' R)-2-(2,3-dicarboxylcyclopropyl)-glycine (1 muM), nor L-serine-O-phosphate (30 muM) (agonists for group II and III mGIuRs, respectively) affectthe NMDA-evoked responses. In striatal medium spiny neurons, NMDA responses are potentiated by endogenous acetylcholine via MI-like muscarinic receptors. Since the enhancement of NMDA responses by 3,5-DHPG and by M1-like muscarinmic agonists was shownto share common post-receptor mechanisms, we verified whether the muscarinic potentiation of NMDA responses was affected in these group I mGluR-deficient mice. Both in mGluR1 and mGluR5 knockout animals, in the presence of either muscarine or the M1-like muscarinic receptor agonist McN-A-343, the positive modulation of the NMDA-induced membrane depolarization persisted. These results confirm the permissive role of group I mGluRs on NMDA responses in striatal neurons and reveal that this functional interplay occurs exclusively through the mGluR5 subtype, The NMDA-mGluR5 interaction might play an important modulatory role in the final excitatory drive from corticostriatal afferents and suggests that drugs acting at mGluR5 might prove useful for the treatment of movement disorders involving the striatum. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 02/12/20 alle ore 14:51:56