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Titolo:
Active erk regulates microtubule stability in H-ras-transformed cells
Autore:
Harrison, RE; Turley, EA;
Indirizzi:
Univ Toronto, Dept Anat & Cell Biol, Toronto, ON M5G 1X8, Canada Univ Toronto Toronto ON Canada M5G 1X8 Biol, Toronto, ON M5G 1X8, Canada Hosp Sick Children, Div Cardiovasc Res, Toronto, ON M5G 1X8, Canada Hosp Sick Children Toronto ON Canada M5G 1X8 Toronto, ON M5G 1X8, Canada
Titolo Testata:
NEOPLASIA
fascicolo: 5, volume: 3, anno: 2001,
pagine: 385 - 394
SICI:
1522-8002(200109/10)3:5<385:AERMSI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BREAST-CANCER; PROTEIN-KINASE; MAP KINASE; ALPHA-TUBULIN; DYNAMICS; PATHWAY; PHOSPHORYLATION; ASSOCIATION; EXPRESSION; INVASION;
Keywords:
erk; ras oncogene; microtubule stability; breast epithelia fibroblast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Turley, EA London Reg Canc Ctr, Canc Res Labs, 790 Commissioners Rd E, London, ON N6A4L6, Canada London Reg Canc Ctr 790 Commissioners Rd E London ONCanada N6A 4L6
Citazione:
R.E. Harrison e E.A. Turley, "Active erk regulates microtubule stability in H-ras-transformed cells", NEOPLASIA, 3(5), 2001, pp. 385-394

Abstract

Increasing evidence suggests that activated erk regulates cell functions, at least in part, by mechanisms that do not require gene transcription. Here we show that the map kinase, erk, decorates microtubules (MTs) and mitotic spindles in both parental and mutant active ras-transfected 10T1/2 fibroblasts and MCF10A breast epithelial cells. Approximately 20% of total cellular erk decorated MTs in both cell lines. A greater proportion of activated erk was associated with MTs in the presence of mutant active H-ras than in parental cells. Activation of erk by the ras pathway coincided with a decrease in the stability of MT, as detected by a stability marker. The MKK1 inhibitor, PD98059 and transfection of a dominant negative MKK1 blocked ras-induced instability of MTs but did not modify the association of erk with MTsor affect MT stability of the parental cells. These results indicate that the subset of active erk kinase that associates with MTs contributes to their instability in the presence of a mutant active ras. The MT-associated subset of active erk likely contributes to the enhanced invasive and proliferative abilities of cells containing mutant active H-ras.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/12/19 alle ore 14:41:23