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Titolo:
Phenotype of FAECB (Facility for Automated Experiments in Cell Biology) Chinese hamster ovary mutants with minimal UV-sensitivity
Autore:
Busch, DB; Ziffer, DW; Coleman, D; Wills, L; McDonough, HG; Jones, NJ;
Indirizzi:
Univ Liverpool, Donnan Labs, Sch Biol Sci, Liverpool L69 7ZD, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 7ZD , Merseyside, England Armed Forces Inst Pathol, Washington, DC 20306 USA Armed Forces Inst Pathol Washington DC USA 20306 Washington, DC 20306 USA
Titolo Testata:
MUTATION RESEARCH-DNA REPAIR
fascicolo: 1-2, volume: 487, anno: 2001,
pagine: 31 - 39
SICI:
0921-8777(20011101)487:1-2<31:POF(FA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-DAMAGING AGENTS; COMPLEMENTATION GROUP; GENETIC-ANALYSIS; EXCISION-REPAIR; CHO CELLS; ATAXIA-TELANGIECTASIA; CROSS-SENSITIVITY; ANEMIA CELLS; DEFICIENT; LINE;
Keywords:
DNA repair; mutants; rodent; ultraviolet light; Xeroderma pigmentosum; Fanconi anemia; ERCC1; XRCC1; XRCC9; XPV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Jones, NJ Univ Liverpool, Donnan Labs, Sch Biol Sci, Liverpool L69 7ZD, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 7ZD de, England
Citazione:
D.B. Busch et al., "Phenotype of FAECB (Facility for Automated Experiments in Cell Biology) Chinese hamster ovary mutants with minimal UV-sensitivity", MUT R-DNA R, 487(1-2), 2001, pp. 31-39

Abstract

The Facility for Automated Experiments in Cell Biology (FAECB) collection of over 200 lines of ultraviolet (UV)-sensitive mutant Chinese hamster ovary (CHO) cells has previously been studied for complementation group assignment (CG), with representatives of rodent UV CGs 1-6 (ERCC1-6) and the new rodent XRCC9/FANCG group identified. Ten mutants from the collection, including a further six derived from wildtype AA8, three UV-sensitive double-mutants of CHO ERCC1 cell line UV4, and a UV-sensitive mutant of CHO XRCC1 cellline EM9, had not been assigned or characterized in these previous studies. These 10 mutants include 8 with approximately 1.5-fold the UV-sensitivityof the parental line (AAS, EM9, or UV4), and 2 with about 2-fold the UV-sensitivity of AAS. The present study reports the partial characterization ofthese 10 mutants in terms of sensitivity to UV (with and without caffeine), ionizing radiation, mitomycin C (MMC) and ethyl methanesulfonate (EMS); proficiency in DNA repair (unscheduled DNA synthesis (UDS)); and UV-mutability. The phenotypes of the 10 cell lines were heterogeneous, a number showedreduced UDS or UV-sensitization by caffeine, whilst others showed marked sensitivity to EMS or MMC, and they may have mutations in different genes involved in nucleotide excision repair, post-replicational repair, base excision repair or recombinational repair. Previous mutants isolated as part of the FAECB collection have proved to be extremely important in characterizing mammalian DNA repair processes and cloning human repair genes and these current mutants, whilst not as hypersensitive to UV, may still have the potential to make further contributions. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 19/09/20 alle ore 16:37:17