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Titolo:
Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein
Autore:
Deml, L; Bojak, A; Steck, S; Graf, M; Wild, J; Schirmbeck, R; Wolf, H; Wagner, R;
Indirizzi:
Univ Regensburg, Inst Med Microbiol, D-93053 Regensburg, Germany Univ Regensburg Regensburg Germany D-93053 , D-93053 Regensburg, Germany Univ Ulm, Inst Med Microbiol & Immunol, D-89069 Ulm, Germany Univ Ulm Ulm Germany D-89069 d Microbiol & Immunol, D-89069 Ulm, Germany
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 22, volume: 75, anno: 2001,
pagine: 10991 - 11001
SICI:
0022-538X(200111)75:22<10991:MEOCUO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-LYMPHOCYTE RESPONSES; LONG-TERM NONPROGRESSOR; IMMUNE-RESPONSE; CPG MOTIFS; HIV-1; IMMUNIZATION; GENE; INFECTION; CELLS; SEQUENCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Wagner, R Klinikum Regensburg, Inst Med Microbiol, Franz Josef Strauss Allee 11, D-93053 Regensburg, Germany Klinikum Regensburg Franz Josef Strauss Allee 11 Regensburg Germany D-93053
Citazione:
L. Deml et al., "Multiple effects of codon usage optimization on expression and immunogenicity of DNA candidate vaccines encoding the human immunodeficiency virus type 1 Gag protein", J VIROLOGY, 75(22), 2001, pp. 10991-11001

Abstract

We have analyzed the influence of codon usage modifications on the expression levels and immunogenicity of DNA vaccines, encoding the human immunodeficiency virus type 1 (HIV-1) group-specific antigen (Gag). In the presence of Rev, an expression vector containing the Mid-type (wt) gag gene flanked by essential cis-acting sites such as the 5'-untranslated region and 3'-Revresponse element supported substantial Gag protein expression and secretion in human H1299 and monkey COS-7 cells. However, only weak Gag production was observed from the murine muscle cell line C2C12. In contrast, optimization of the Gag coding sequence to that of highly expressed mammalian genes (syngag) resulted in an obvious increase in the G+C content and a Rev-independent expression and secretion of Gag in all tested mammalian cell lines, including murine C2C12 muscle cells. Mice immunized intramuscularly with the syngag plasmid showed Th1-driven humoral and cellular responses that weresubstantially higher than those obtained after injection of the Rev-dependent wild-type (wt) gag vector system. In contrast, intradermal immunizationof both wt gag and syngag vector systems with the particle gun induced a Th2-biased antibody response and no cytotoxic T lymphocytes. Deletion analysis demonstrated that the CpG motifs generated within syngag by codon optimization do not contribute significantly to the high immunogenicity of the syngag plasmid. Moreover, low doses of coadministered stimulatory phosphorothioate oligodeoxynucleotides (ODNs) had only a weak effect on antibody production, whereas at higher doses immunostimulatory and nonstimulatory ODNs showed a dose-dependent suppression of humoral responses. These results suggest that increased Gag expression, rather than modulation of CpG-driven vector immunity, is responsible for the enhanced immunogenicity of the syngag DNA vaccine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 17:06:37