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Titolo:
C terminus of infectious bursal disease virus major capsid protein VP2 is involved in definition of the T number for capsid assembly
Autore:
Caston, JR; Martinez-Torrecuadrada, JL; Maraver, A; Lombardo, E; Rodriguez, JF; Casal, JI; Carrascosa, JL;
Indirizzi:
CSIC, Dept Estructura Macromol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain CSIC Madrid Spain E-28049 mol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain CSIC, Dept Mol & Cellular Biol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain CSIC Madrid Spain E-28049 iol, Ctr Nacl Biotecnol, E-28049 Madrid, Spain Ingenasa, Madrid 28037, Spain Ingenasa Madrid Spain 28037Ingenasa, Madrid 28037, Spain
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 22, volume: 75, anno: 2001,
pagine: 10815 - 10828
SICI:
0022-538X(200111)75:22<10815:CTOIBD>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; BACULOVIRUS EXPRESSION VECTORS; 3-DIMENSIONAL STRUCTURE; CRYOELECTRON MICROSCOPY; BLUETONGUE VIRUS; ELECTRON CRYOMICROSCOPY; IMAGE-RECONSTRUCTION; ANTIGENIC PROPERTIES; ICOSAHEDRAL VIRUSES; GENOMIC SEGMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
81
Recensione:
Indirizzi per estratti:
Indirizzo: Carrascosa, JL CSIC, Dept Estructura Macromol, Ctr Nacl Biotecnol, Campus UAM,Cantoblanco, E-28049 Madrid, Spain CSIC Campus UAM,Cantoblanco Madrid Spain E-28049 id, Spain
Citazione:
J.R. Caston et al., "C terminus of infectious bursal disease virus major capsid protein VP2 is involved in definition of the T number for capsid assembly", J VIROLOGY, 75(22), 2001, pp. 10815-10828

Abstract

Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is a double-stranded RNA virus. The IBDV capsid is formed by two major structural proteins, VP2 and VP3, which assemble to form a T=13 markedly nonspherical capsid. During viral infection, VP2 is initially synthesized as aprecursor, called VPX, whose C end is proteolytically processed to the mature form during capsid assembly. We have computed three-dimensional maps ofIBDV capsid and virus-like particles built up by NT2 alone by using electron cryomicroscopy and image-processing techniques. The IBDV single-shelled capsid is characterized by the presence of 260 protruding trimers on the outer surface. Five classes of trimers can be distinguished according to their different local environments. When VP2 is expressed alone in insect cells, dodecahedral particles form spontaneously; these may be assembled into larger, fragile icosahedral capsids built up by 12 dodecahedral capsids. Eachdodecahedral capsid is an empty T=1 shell composed of 20 trimeric clustersof NT2. Structural comparison between IBDV capsids and capsids consisting of VP2 alone allowed the determination of the major capsid protein locations and the interactions between them. Whereas NT2 forms the outer protrudingtrimers, VP3 is found as trimers on the inner surface and may be responsible for stabilizing functions. Since elimination of the C-terminal region ofVPX is correlated with the assembly of T=1 capsids, this domain might be involved (either alone or in cooperation with VP3) in the induction of different conformations of VP2 during capsid morphogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:30:13