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Titolo:
Odorant feature detection: Activity mapping of structure response relationships in the zebrafish olfactory bulb
Autore:
Fuss, SH; Korsching, SI;
Indirizzi:
Univ Cologne, Inst Genet, D-50674 Cologne, Germany Univ Cologne Cologne Germany D-50674 nst Genet, D-50674 Cologne, Germany
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 21, volume: 21, anno: 2001,
pagine: 8396 - 8407
SICI:
0270-6474(20011101)21:21<8396:OFDAMO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITRAL TUFTED CELLS; RECEPTOR NEURONS; FUNCTIONAL IDENTIFICATION; GLOMERULAR LAYER; CHANNEL CATFISH; SENSORY MAP; AMINO-ACIDS; EXPRESSION; REPRESENTATIONS; COMBINATORIAL;
Keywords:
olfactory bulb; odorant feature; zebrafish; odorant; olfactory receptor; Danio rerio; Calcium Green; optical imaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Korsching, SI Univ Cologne, Inst Genet, Zulpicher Str 47, D-50674 Cologne,Germany Univ Cologne Zulpicher Str 47 Cologne Germany D-50674 rmany
Citazione:
S.H. Fuss e S.I. Korsching, "Odorant feature detection: Activity mapping of structure response relationships in the zebrafish olfactory bulb", J NEUROSC, 21(21), 2001, pp. 8396-8407

Abstract

The structural determinants of an odor molecule necessary and/or sufficient for interaction with the cognate olfactory receptor(s) are not known. Olfactory receptor neurons expressing the same olfactory receptor converge in the olfactory bulb. Thus, optical imaging of neuronal activity in the olfactory bulb can visualize at once the contributions by all the different olfactory receptors responsive to a particular odorant. We have used this technique to derive estimates about the structural requirements and minimal number of different zebrafish olfactory receptors that respond to a series of naturally occurring amino acids and some structurally related compounds. We report that the alpha -carboxyl group, the alpha -amino group, and L-conformation of the amino acid are all required for activation of amino acid-responsive receptors. Increasing carbon chain length recruits successively more receptors. With increasing concentrations, the activity patterns induced by a homolog series of amino acids became more similar to each other. Atintermediate concentrations patterns were unique across substances and across concentrations. The introduction of a terminal amino group (charged) both recruits additional receptors and prevents binding to some of the receptors that were responsive to the unsubstituted analog. In contrast, the introduction of a beta -hydroxyl group (polar) excluded the odorants from some of the receptors that are capable of binding the unsubstituted analog. Cross-adaptation experiments independently confirmed these results. Thus, odorant detection requires several different receptors even for relatively simple odorants such as amino acids, and individual receptors require the presence of some molecular features, the absence of others, and tolerate still other molecular features.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 18:30:49