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Titolo:
Molecular cloning and expression of woodchuck granulocyte-macrophage colony stimulating factor
Autore:
Wu, HL; Chen, PJ; Lin, HK; Lee, RS; Lin, HL; Liu, CJ; Lee, PJ; Lee, JJ; Chen, DS;
Indirizzi:
Natl Taiwan Univ Hosp, Hepat Res Ctr, Taipei, Taiwan Natl Taiwan Univ Hosp Taipei Taiwan Hosp, Hepat Res Ctr, Taipei, Taiwan Natl Taiwan Univ, Coll Med, Grad Inst Clin Med & Microbiol, Taipei, TaiwanNatl Taiwan Univ Taipei Taiwan nst Clin Med & Microbiol, Taipei, Taiwan Natl Taiwan Univ Hosp, Dept Internal Med, Taipei 100, Taiwan Natl Taiwan Univ Hosp Taipei Taiwan 100 Internal Med, Taipei 100, Taiwan Natl Taiwan Univ, Coll Med, Lab Anim Ctr, Taipei, Taiwan Natl Taiwan UnivTaipei Taiwan , Coll Med, Lab Anim Ctr, Taipei, Taiwan Natl Taiwan Univ, Dept Vet Med, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 Dept Vet Med, Taipei 10764, Taiwan
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 3, volume: 65, anno: 2001,
pagine: 567 - 575
SICI:
0146-6615(200111)65:3<567:MCAEOW>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-B VIRUS; BIOLOGICAL-ACTIVITY; LANGERHANS CELLS; MESSENGER-RNA; GM-CSF; PURIFICATION; DNA; VACCINATION; CARCINOMA; SEQUENCES;
Keywords:
woodchuck; woodchuck hepatitis virus; cloning; granulocyte macrophage colony stimulating factor; chronic hepatitis B;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, DS Natl Taiwan Univ Hosp, Hepat Res Ctr, 7 Chung Shan SRd, Taipei, Taiwan Natl Taiwan Univ Hosp 7 Chung Shan SRd Taipei Taiwan ei, Taiwan
Citazione:
H.L. Wu et al., "Molecular cloning and expression of woodchuck granulocyte-macrophage colony stimulating factor", J MED VIROL, 65(3), 2001, pp. 567-575

Abstract

Granulocyte-macrophage colony stimulating factor (GM-CSF) has immunoregulatory and antiviral effects, and may thus be promising for the treatment of chronic hepatitis B. Using woodchuck hepatitis virus (WHV)-infected woodchuck as an animal model to test the efficacy and safety of GM-CSF on the therapy of chronic hepatitis B, woodchuck GM-CSF will be require due to the apparent species-specific activity of GM-CSF. The cDNA of woodchuck GM-CSF wascloned using reverse transcription-polymerase chain reaction (RT-PCR) withprimers deriving from highly conserved regions of GM-CSF genes from other species. The deduced amino acids including the signal peptide, is 138 in length and its identities to human, murine, canine and bovine GM-CSFs are 63,49, 63, and 63% respectively. The genomic DNA of woodchuck GM-CSF was alsocloned by PCR. Its organization is highly homologous to that of human and murine GM-CSF genes, consisting of four exons and three introns. Cloned woodchuck GM-CSF was expressed transiently in 293T cells. The recombinant protein expressed was found to stimulate the growth and differentiation of woodchuck bone marrow cells, indicating the protein expressed by the cloned gene is functional. These results pave the way for future studies on the potential role of GM-CSF for the treatment of chronic hepatitis B by using this animal model.

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Documento generato il 04/07/20 alle ore 13:54:40